Rodbard D, Chrambach A
Proc Natl Acad Sci U S A. 1970 Apr;65(4):970-7. doi: 10.1073/pnas.65.4.970.
Unified theory for gel electrophoresis and gel filtration: The behavior of macromolecules in gel filtration and gel electrophoresis may be predicted from Ogston's model for a random meshwork of fibers. This model has been generalized to apply to nonspherical molecules and to several gel types. The model provides equations for inter-relationships between mobility, partition coefficient, gel concentration, and molecular radius; it gives a non-Gaussian distribution of pore sizes as a function of gel concentration. The theory defines conditions for optimal separation and optimal resolution in gel filtration and gel electrophoresis. The difference in resolving power between the two fractionation methods is accounted for by the fact that gel filtration is a form of partition chromatography.
凝胶过滤和凝胶电泳中大分子的行为可以根据奥格斯顿（Ogston）的纤维随机网络模型来预测。该模型已被推广应用于非球形分子和多种凝胶类型。该模型提供了迁移率、分配系数、凝胶浓度和分子半径之间相互关系的方程；它给出了孔径随凝胶浓度变化的非高斯分布。该理论定义了凝胶过滤和凝胶电泳中最佳分离和最佳分辨率的条件。两种分离方法在分辨能力上的差异是由于凝胶过滤是分配色谱的一种形式这一事实造成的。
