Watson B K, Kunz L J, Moellering R C
J Clin Microbiol. 1975 Mar;1(3):268-73. doi: 10.1128/jcm.1.3.268-273.1975.
This paper deals with the fluorescent antibody (FA) method for identifying six commonly occuring and two rare groups of streptococci by using commercially prepared (Difco) conjugates. We have shown that group-specific FA produced frequent cross-reactions with heterologous groups of organisms. These reactions varied with different strains of the same serogroup. Nonetheless, there was distinct overall patterns in the intensity and appearance of the homologous and heterologous reactions. When monitored by the precipitin test with Rantz and Randall extracts, these patterns led to the correct identification of 90 to 100% of specimens of serogroup A, B, C, and G streptococci. Many members of groups D and F also showed distinctive reaction patterns. However, there was a significant number of strains of both groups D and F that either failed to strain or stained poorly with the homologous conjugate. As a result, the identification of these serogroups by FA was less reliable.
本文论述了使用市售（迪夫科）结合物通过荧光抗体（FA）法鉴定六种常见和两种罕见的链球菌群。我们已经表明，群特异性FA经常与异源生物群发生交叉反应。这些反应因同一血清群的不同菌株而异。尽管如此，同源和异源反应的强度和表现仍有明显的总体模式。当用兰茨和兰德尔提取物进行沉淀试验监测时，这些模式能正确鉴定90%至100%的A、B、C和G血清群链球菌标本。D群和F群的许多成员也表现出独特的反应模式。然而，D群和F群都有相当数量的菌株要么未染色，要么用同源结合物染色不佳。因此，通过FA鉴定这些血清群的可靠性较低。
