Kordi Negin, Azizi Mohammad, Samadi Mohammad, Tahmasebi Worya
Department of Exercise Physiology, Faculty of Sport Sciences, Razi University, Kermanshah, Iran.
Exercise Physiology Research Center, Lifestyle Institute, Baqiyatallah University of Medical Sciences, Tehran, Iran.
Sci Rep. 2025 Oct 23;15(1):37132. doi: 10.1038/s41598-025-21130-2.
Previous studies have shown that regular physical activity combined with the use of the superfood NBS supplement can reduce the cardiac damage caused by methamphetamine. However, the genetic mechanisms affecting apoptosis in the myocardium affected by these methods have not been fully identified. The aim of this research was to examine the impact of aerobic training and NBS superfood intake on apoptosis and cardiac damage in methamphetamine- withdrawn rats. 104 male Wistar rats were randomly assigned to eight distinct groups. Methamphetamine was given intraperitoneally at a dose of 10 mg/kg twice a day during the first week. The dosage was then steadily raised by 1 mg/kg every week from the second to the sixth week. The training plan included five days a week, 60 min of moderate-intensity treadmill aerobic training across six weeks. Moreover, for a duration of 45 days, the NBS Superfood supplement was given daily at a dose of 25 g/kg. Myocardial sampling was performed to measure the levels of Melusin and caspase-3 gene transcripts, while blood serum analysis was performed to examine the concentrations of creatine kinase (CK) and lactate dehydrogenase (LDH) enzymes. Data analysis utilized a one-way analysis of variance (p ≤ 0.05). The results show that methamphetamine use greatly raised caspase-3 gene expression as well as CK and LDH levels while simultaneously lowering Melusin gene expression (p ≤ 0.05). Furthermore, lowering serum CK and LDH levels, enhancing Melusin gene expression, and lowering caspase-3 gene expression were all accomplished through aerobic training, nutritional supplementation, and the synergistic intervention (p 0.05). The results of the study showed that the combined intervention (training and supplementation) had better effects than the single intervention (exercise alone or supplementation alone). This was manifested in a significant decrease in caspase-3 levels and a significant increase in Melusin expression. The results of this study indicate that combining endurance training with the NBS Superfood supplement NBS can modulate genetic changes associated with methamphetamine-induced cell death and myocardial damage. This combined approach can be proposed as a novel method for addiction cessation in animal studies that can pave the way for future research in human populations.
先前的研究表明，规律的体育活动与食用超级食物NBS补充剂相结合，可以减少甲基苯丙胺对心脏造成的损害。然而，这些方法影响受甲基苯丙胺影响的心肌细胞凋亡的遗传机制尚未完全明确。本研究的目的是探讨有氧训练和摄入NBS超级食物对甲基苯丙胺戒断大鼠心肌细胞凋亡和心脏损伤的影响。104只雄性Wistar大鼠被随机分为8个不同的组。在第一周，每天两次以10毫克/千克的剂量腹腔注射甲基苯丙胺。从第二周到第六周，剂量每周稳步增加1毫克/千克。训练计划包括每周五天，为期六周，每次60分钟的中等强度跑步机有氧训练。此外，持续45天，每天以25克/千克的剂量给予NBS超级食物补充剂。采集心肌样本以测量Melusin和半胱天冬酶-3基因转录本的水平，同时进行血清分析以检测肌酸激酶（CK）和乳酸脱氢酶（LDH）的浓度。数据分析采用单因素方差分析（p≤0.05）。结果表明，使用甲基苯丙胺显著提高了半胱天冬酶-3基因表达以及CK和LDH水平，同时降低了Melusin基因表达（p≤0.05）。此外，通过有氧训练、营养补充和协同干预均实现了降低血清CK和LDH水平、增强Melusin基因表达以及降低半胱天冬酶-3基因表达（p<0.05）。研究结果表明，联合干预（训练和补充）比单一干预（单独运动或单独补充）效果更好。这表现为半胱天冬酶-3水平显著降低和Melusin表达显著增加。本研究结果表明，耐力训练与NBS超级食物补充剂NBS相结合可以调节与甲基苯丙胺诱导的细胞死亡和心肌损伤相关的基因变化。这种联合方法可以作为动物研究中一种新的戒毒方法提出，为未来在人类群体中的研究铺平道路。
