编码痢疾志贺氏菌主要外膜蛋白OmpA的核苷酸序列。
The nucleotide sequence coding for major outer membrane protein OmpA of Shigella dysenteriae.
作者信息
Braun G, Cole S T
出版信息
Nucleic Acids Res. 1982 Apr 10;10(7):2367-78. doi: 10.1093/nar/10.7.2367.
Abstract
The nucleotide sequence of the ompA gene from Shigella dysenteriae has been determined and the amino acid sequence of the pro-OmpA protein predicted. Sequence comparison between the ompA genes of S.dysenteriae and Escherichia coli showed that features such as mRNA secondary structure and codon usage, as well as polypeptide function, have been conserved during evolution. The pro-OmpA protein of S.dysenteriae consists of 351 residues, as opposed to the 346 of the E.coli protein and also shows several amino acid changes. These changes have been used to interpret differences in the biological activity of the two proteins.
摘要
已确定痢疾志贺氏菌ompA基因的核苷酸序列，并预测了前OmpA蛋白的氨基酸序列。痢疾志贺氏菌和大肠杆菌ompA基因之间的序列比较表明，mRNA二级结构、密码子使用以及多肽功能等特征在进化过程中得以保留。痢疾志贺氏菌的前OmpA蛋白由351个残基组成，而大肠杆菌蛋白为346个残基，并且还存在几个氨基酸变化。这些变化已被用于解释两种蛋白质生物活性的差异。
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