Kelly M T, Brashear R E, Martin R R, White A
Infect Immun. 1971 Sep;4(3):228-31. doi: 10.1128/iai.4.3.228-231.1971.
Mixed leukocyte suspensions, containing 10(6) leukocytes per ml, were prepared from dog peripheral blood by dextran sedimentation. Lysates of these suspensions, prepared by sonification, were administered intravenously to the autologous dogs. The plasma histamine levels were increased to an average value 320% above the control values (P < 0.001). The individual elements in the mixed leukocyte suspensions were separated by sucrose gradient and glass bead column fractionation procedures, and purified suspensions of platelets (no other formed elements present), lymphocytes (95 to 100%), and granulocytes (90 to 98%) were obtained. Lysates of these suspensions all had significant histamine-releasing activity when administered intravenously to dogs. Although leukocytes and platelets from other species are known to harbor mediators of histamine release, this study provides the first demonstration of the histamine-releasing activity of dog leukocytes and platelets. Many of the studies of leukocyte and platelet histamine-releasing factors have employed in vitro systems leaving the in vivo role of these factors in doubt. The present studies provide evidence that leukocyte and platelet histamine-releasing factors have in vivo activity.
通过葡聚糖沉降法从犬外周血制备每毫升含10⁶个白细胞的混合白细胞悬液。通过超声处理制备这些悬液的裂解物，并将其静脉注射给自体犬。血浆组胺水平升高至比对照值平均高320%（P<0.001）。通过蔗糖梯度和玻璃珠柱分级分离程序分离混合白细胞悬液中的各个成分，获得了血小板（不存在其他有形成分）、淋巴细胞（95%至100%）和粒细胞（90%至98%）的纯化悬液。当将这些悬液的裂解物静脉注射给犬时，它们都具有显著的组胺释放活性。虽然已知其他物种的白细胞和血小板含有组胺释放介质，但本研究首次证明了犬白细胞和血小板的组胺释放活性。许多关于白细胞和血小板组胺释放因子的研究采用了体外系统，使得这些因子在体内的作用存在疑问。本研究提供了证据表明白细胞和血小板组胺释放因子具有体内活性。
