通过电聚焦法分离金黄色葡萄球菌产生的两种淋巴细胞促有丝分裂原。
Isolation by electrofocusing of two lymphocyte mitogens produced by Staphylococcus aureus.
作者信息
Kreger A S, Cuppari G, Taranta A
出版信息
Infect Immun. 1972 May;5(5):723-7. doi: 10.1128/iai.5.5.723-727.1972.
Staphylococcus aureus strain DA352, grown in a diffusate of Todd-Hewitt broth, produced two extracellular nondialyzable lymphocyte mitogens having isoelectric points of 5.5 to 5.7 and 8.6 to 9.0. The mitogens were separable from one another by isoelectric focusing and could be isolated free of detectable amounts of other staphylococcal products by ethanol precipitation followed by isoelectric focusing. Dose-response curves with both mitogens showed a maximum per cent transformation in the range (90%+) obtained with phytohemagglutinin (PHA), a decrease of transformation with excess mitogen, and, with decreasing concentrations, a slope somewhat less steep than that obtained with PHA. Incubation with undigested or pepsin-digested pooled human gamma globulin enhanced the activity of the basic mitogen.
金黄色葡萄球菌菌株DA352在托德-休伊特肉汤的扩散液中生长，产生了两种细胞外不可透析的淋巴细胞有丝分裂原，其等电点分别为5.5至5.7和8.6至9.0。通过等电聚焦可将这两种有丝分裂原彼此分离，并且通过乙醇沉淀继以等电聚焦，可分离得到不含可检测量其他葡萄球菌产物的有丝分裂原。两种有丝分裂原的剂量反应曲线显示，在与植物血凝素（PHA）相同的范围内（90%以上）有最大转化率，过量有丝分裂原会使转化率降低，且随着浓度降低，斜率比PHA的斜率略平缓。与未消化或经胃蛋白酶消化的混合人γ球蛋白一起孵育可增强碱性有丝分裂原的活性。
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