可溶性和不溶性葡萄球菌蛋白A（SPA）的不同促有丝分裂活性。

Different mitogenic activity of soluble and insoluble staphylococcal protein A (SPA).

作者信息

Romagnani S, Amadori A, Giudizi M G, Biagiotti R, Maggi E, Ricci M

出版信息

Immunology. 1978 Sep;35(3):471-8.

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1457639/

Abstract

The response to SPA and Staphylococcus strain Cowan I (StaCw) of highly purified populations of peripheral blood and tonsil human lymphocytes was investigated. Purified T lymphocytes isolated from perpheral blood by E-rosetting were unable to respond in vitro to StaCw. Highly purified B-cell populations from tonsils did not show any proliferative response in the presence of soluble SPA. The addition to highly purified B-cell suspensions from human tonsils of increasing concentrations of autologous T lymphocytes did not induce any increase of thymidine uptake in the presence of StaCw. However, it was able to restore a marked proliferative response of the B-cell cultures to soluble SPA, even though mitomycin-treated T lymphocytes were added. The low response of highly purified peripheral blood T lymphocytes to soluble SPA could be potentiated by the addition of autologous mitomycin-treated B cells, whereas the unresponsiveness of purified T lymphocytes to StaCw was not affected. Mitogenic activity of SPA coupled to Sepharose beads was different from that of soluble SPA and paralleled that of StaCw. These data strongly suggest that insoluble SPA is a T-cell-independent B-cell mitogen in man, whereas soluble SPA, like PWM, exerts its activity on B cells only in the presence of T cells.

摘要

研究了外周血和扁桃体人淋巴细胞高度纯化群体对葡萄球菌A蛋白（SPA）和考恩I型葡萄球菌菌株（StaCw）的反应。通过E花环法从外周血中分离出的纯化T淋巴细胞在体外对StaCw无反应。来自扁桃体的高度纯化B细胞群体在可溶性SPA存在下未显示任何增殖反应。在人扁桃体高度纯化的B细胞悬液中加入浓度递增的自体T淋巴细胞，在StaCw存在下并未诱导胸苷摄取增加。然而，即使加入经丝裂霉素处理的T淋巴细胞，它也能够恢复B细胞培养物对可溶性SPA的显著增殖反应。加入自体经丝裂霉素处理的B细胞可增强高度纯化的外周血T淋巴细胞对可溶性SPA的低反应性，而纯化T淋巴细胞对StaCw的无反应性不受影响。与琼脂糖珠偶联的SPA的促有丝分裂活性不同于可溶性SPA，且与StaCw的相似。这些数据强烈表明，不溶性SPA是人类中一种不依赖T细胞的B细胞有丝分裂原，而可溶性SPA与美洲商陆有丝分裂原（PWM）一样，仅在T细胞存在时才对B细胞发挥其活性。

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本文引用的文献

1

Cellular reactions with protein A of Staphylococcus aureus.

Proc Soc Exp Biol Med. 1972 Feb;139(2):480-3. doi: 10.3181/00379727-139-36168.

2

Response of peripheral blood lymphocytes from normal, hypogammaglobulinemic and chronic lymphocytic leukemic patients.正常、低丙种球蛋白血症和慢性淋巴细胞白血病患者外周血淋巴细胞的反应。

J Immunol. 1972 Jan;108(1):178-82.

3

Movement of IgG receptors on the lymphocyte surface induced by protein A of Staphylococcus aureus.金黄色葡萄球菌蛋白A诱导淋巴细胞表面IgG受体的移动

Immunology. 1974 Jun;26(6):1081-91.

4

Rosette formation by mouse lymphocytes. II. T-cell specificities in a CRL subpopulation.小鼠淋巴细胞的玫瑰花结形成。II. CRL亚群中的T细胞特异性。

Clin Exp Immunol. 1974 Oct;18(2):177-86.

5

Effect of anti-lymphocyte serum on responses of human peripheral-blood lymphocytes to specific and non-specific stimulants in vitro.

Lancet. 1967 Dec 23;2(7530):1317-9. doi: 10.1016/s0140-6736(67)90909-9.

6

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J Immunol. 1966 Dec;97(6):822-7.

7

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8

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9

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