Shan Fangjian, Heng Xingyu, Yao Lihua, Xu Guichuan, Hu Jun, Pan Xiangqiang, Chen Gaojian
State and Local Joint Engineering Laboratory for Novel Functional Polymeric Materials, Jiangsu Key Laboratory of Advanced Functional Polymer Materials, College of Chemistry, Chemical Engineering and Materials Science, Soochow University Suzhou 215123 P. R. China
School of Chemistry and Chemical Engineering, Southeast University Nanjing 211189 P. R. China.
Chem Sci. 2025 Oct 29;16(48):23047-23054. doi: 10.1039/d5sc05541e. eCollection 2025 Dec 10.
Cell surface engineering plays a crucial role in enhancing the functionality and therapeutic potential of living cells through precise and controllable surface modifications. However, traditional conjugation strategies often suffer from limited efficiency or inherent complexity. In this work, we developed a selenol-yne click (SYC) reaction for efficient and rapid cell surface functionalization, enabling covalent attachment of diverse payloads to a variety of cell types. The SYC reaction enables highly efficient conjugation of fluorescent molecules, micron-scale "backpack" particles, and functional polymers onto cell surfaces, including HeLa, B16-OVA, and Jurkat T cells. Drug-loaded backpacks carried by the cells demonstrated effective drug delivery to target cells. Moreover, cell surface glycosylation this method enables efficient modulation of cell-cell interactions and immune responses. Importantly, glycosylation was also successfully achieved in living zebrafish through the SYC reaction, demonstrating its potential for applications. This SYC-based strategy offers a fast and versatile platform for advancing cell surface engineering.
细胞表面工程通过精确且可控的表面修饰，在增强活细胞的功能和治疗潜力方面发挥着关键作用。然而，传统的偶联策略往往效率有限或固有复杂性高。在这项工作中，我们开发了一种硒醇-炔点击（SYC）反应，用于高效快速的细胞表面功能化，使各种有效负载能够共价连接到多种细胞类型上。SYC反应能够将荧光分子、微米级“背包”颗粒和功能聚合物高效偶联到细胞表面，包括HeLa细胞、B16-OVA细胞和Jurkat T细胞。细胞携带的载药背包显示出对靶细胞的有效药物递送。此外，通过这种方法进行的细胞表面糖基化能够有效调节细胞间相互作用和免疫反应。重要的是，通过SYC反应在活体斑马鱼中也成功实现了糖基化，证明了其在体内应用的潜力。这种基于SYC的策略为推进细胞表面工程提供了一个快速且通用的平台。
