通过二价阳离子对细胞核进行分级分离。核膜的分离。
Fractionation of the nucleus by divalent cations. Isolation of nuclear membranes.
作者信息
Monneron A, Blobel G, Palade G E
出版信息
J Cell Biol. 1972 Oct;55(1):104-25. doi: 10.1083/jcb.55.1.104.
Abstract
0.3-0.5 M MgCl(2) was used to disassemble nuclei and to isolate by a single centrifugation in less than 3 hr a nuclear envelope fraction in 55-60% yield as assessed by phospholipid recovery. Its gross chemical composition was determined and its morphology was studied electron microscopically by sectioning, freeze etching, and negative staining procedures.
摘要
使用0.3 - 0.5M的氯化镁来拆解细胞核，并通过单次离心在不到3小时内分离出核膜组分，根据磷脂回收率评估，产率为55 - 60%。测定了其总体化学成分，并通过切片、冷冻蚀刻和负染色程序用电镜研究了其形态。
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