通过等电聚焦分离的黄病毒结构蛋白的抗原特性分析
Antigenic characterization of flavivirus structural proteins separated by isoelectric focusing.
作者信息
Trent D W
出版信息
J Virol. 1977 Jun;22(3):608-18. doi: 10.1128/JVI.22.3.608-618.1977.
Abstract
Isoelectrofocusing of nonionic-detergent-disrupted flaviviruses separated the envelope glycoprotein of 53,000 to 58,000 daltons and the nucleocapsid protein of 14,000 daltons. The envelope protein and nucleocapsid protein were isolated at isoelectric points of pI 7.8 and 10.3, respectively. The antigenic determinants of St. Louis encephalitis, Japanese encephalitis, and dengue virus envelope and nucleocapsid proteins were examined by solid-phase competition radioimmunoassay. By the appropriate selection of antiserum and competing proteins, it was possible to distinguish type-specific, complex-reactive and flavivirus group-reactive antigenic determinants. The envelope glycoproteins of St. Louis encephalitis, Japanese encephalitis, and dengue viruses were found to contain each of these three classes of antigenic determinants. Most of the determinants on the envelope protein were type specific, some were complex reactive, and a small fraction were flavivirus group reactive. The nucleocapsid protein contained only flavivirus group-reactive antigenic determinants.
摘要
用非离子去污剂裂解黄病毒后进行等电聚焦，分离出了分子量为53,000至58,000道尔顿的包膜糖蛋白和分子量为14,000道尔顿的核衣壳蛋白。包膜蛋白和核衣壳蛋白分别在等电点pI 7.8和10.3处分离得到。通过固相竞争放射免疫测定法检测了圣路易斯脑炎病毒、日本脑炎病毒和登革病毒包膜蛋白及核衣壳蛋白的抗原决定簇。通过适当选择抗血清和竞争蛋白，可以区分型特异性、复合反应性和黄病毒属反应性抗原决定簇。发现圣路易斯脑炎病毒、日本脑炎病毒和登革病毒的包膜糖蛋白均含有这三类抗原决定簇。包膜蛋白上的大多数决定簇是型特异性的，有些是复合反应性的，一小部分是黄病毒属反应性的。核衣壳蛋白仅含有黄病毒属反应性抗原决定簇。
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