Ayoub E M, Dudding B A
J Exp Med. 1973 Jul 1;138(1):117-29. doi: 10.1084/jem.138.1.117.
A temperature-dependent alteration in the synthesis of the group-specific polysaccharide was found to occur in two "variant" streptococcal strains, A-486-Var and C 121/46/4. These strains synthesize a polysaccharide with variant immunochemical characteristics when grown at 37 degrees C. However, when these organisms are grown at lower temperatures, 22 degrees C, an enhanced synthesis of Group A carbohydrate occurs. Other variant strains show no appreciable alteration of the cell wall carbohydrate composition when grown at lower temperatures. Studies on an intermediate strain show that this organism has a propensity for the synthesis of a polysaccharide with higher glucosamine content and enhanced Group A serological reactivity when grown at 22 degrees C. Immunochemical studies performed on the carbohydrates produced by the A-486-Var at various temperatures revealed that the appearance of Group A serological reactivity at lower temperatures is due to the additional synthesis of a polysaccharide with Group A specificity along with the continued synthesis of a variant carbohydrate. This finding contrasts with data obtained on the carbohydrate produced by the intermediate organisms that appears to consist predominently of one molecule bearing dual A and variant antigenic determinants.
在两种“变异”链球菌菌株A - 486 - Var和C 121/46/4中发现了与温度相关的群特异性多糖合成变化。当在37℃生长时，这些菌株合成具有变异免疫化学特性的多糖。然而，当这些生物体在较低温度（22℃）下生长时，A群碳水化合物的合成会增强。其他变异菌株在较低温度下生长时，细胞壁碳水化合物组成没有明显变化。对一种中间菌株的研究表明，该生物体在22℃生长时倾向于合成具有较高葡萄糖胺含量和增强的A群血清学反应性的多糖。对A - 486 - Var在不同温度下产生的碳水化合物进行的免疫化学研究表明，在较低温度下A群血清学反应性的出现是由于额外合成了具有A群特异性的多糖以及变异碳水化合物的持续合成。这一发现与在中间生物体产生的碳水化合物上获得的数据形成对比，后者似乎主要由一个带有双重A和变异抗原决定簇的分子组成。
