Karkas J D, Stavrianopoulos J G, Chargaff E
Proc Natl Acad Sci U S A. 1972 Feb;69(2):398-402. doi: 10.1073/pnas.69.2.398.
Experiments indicating the ability of the ribo strand of a DNA-RNA template to guide polydeoxynucleotide synthesis by highly purified DNA polymerase I of E. coli (EC 2.7.7.7) are presented. With poly(rA).poly(dT) as template, poly(dT) is formed with a high efficiency, but almost no poly(dA). The specific activity of the enzyme, when tested with this template under suitable conditions, is eight times greater than that found for the poly(dA-dT) template. Single-stranded DNA fractions, with no template activity for DNA polymerase, are converted to efficient templates after their transcription by RNA polymerase. A concerted polymerization reaction, in which the action of DNA polymerase is dependent on that of RNA polymerase, can also be demonstrated with synthetic polydeoxynucleotides and single-stranded fractions of denatured DNA as templates.
本文展示了一些实验，这些实验表明DNA - RNA模板的核糖链能够引导由大肠杆菌高度纯化的DNA聚合酶I（EC 2.7.7.7）进行多脱氧核苷酸合成。以聚（rA）·聚（dT）作为模板时，能高效形成聚（dT），但几乎不形成聚（dA）。在合适条件下用该模板测试时，酶的比活性比用聚（dA - dT）模板时高八倍。对DNA聚合酶没有模板活性的单链DNA组分，在经RNA聚合酶转录后会转变为有效的模板。使用合成多脱氧核苷酸和变性DNA的单链组分作为模板，也能证明一种协同聚合反应，其中DNA聚合酶的作用依赖于RNA聚合酶的作用。
