Jonakait G M, Wolf J, Cochard P, Goldstein M, Black I B
Proc Natl Acad Sci U S A. 1979 Sep;76(9):4683-6. doi: 10.1073/pnas.76.9.4683.
To define the fate of embryonic neuroblasts in rat gut, which transiently express several noradrenergic traits, we investigated the high-affinity uptake of norepinephrine. At 12.5 days of gestation, these cells exhibited immunoreactivity to tyrosine hydroxylase [tyrosine 3-monoxygenase; L-tyrosine, tetrahydropteridine:oxygen oxidoreductase (3-hydroxylating), EC 1.14.16.2] and endogenous catecholamine fluorescence. However, by 13.5 days these noradrenergic neurotransmitter phenotypic characters essentially disappeared. In contrast, norepinephrine uptake, which was also apparent at 12.5 days, persisted at least through 17.5 days. These observations indicate that norepinephrine uptake develops as an additional noradrenergic characteristic in these cells and persists after the disappearance of other noradrenergic traits. Consequently, neurotransmitter phenotypic characters may be transiently displayed during normal development in vivo.
为了确定大鼠肠道中短暂表达多种去甲肾上腺素能特征的胚胎神经母细胞的命运，我们研究了去甲肾上腺素的高亲和力摄取。在妊娠12.5天时，这些细胞对酪氨酸羟化酶[酪氨酸3-单加氧酶；L-酪氨酸，四氢蝶呤：氧氧化还原酶（3-羟化），EC 1.14.16.2]表现出免疫反应性以及内源性儿茶酚胺荧光。然而，到13.5天时，这些去甲肾上腺素能神经递质表型特征基本消失。相比之下，在12.5天时也很明显的去甲肾上腺素摄取至少持续到17.5天。这些观察结果表明，去甲肾上腺素摄取作为这些细胞中一种额外的去甲肾上腺素能特征发展而来，并在其他去甲肾上腺素能特征消失后持续存在。因此，神经递质表型特征可能在体内正常发育过程中短暂显示。
