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诺氏疟原虫感染的红细胞中膜糖蛋白的标记

Labelling of membrane glycoprotein in erythrocytes infected with Plasmodium knowlesi.

作者信息

Trigg P I, Hirst S I, Shakespeare P G, Tappenden L

出版信息

Bull World Health Organ. 1977;55(2-3):205-9.

Abstract

Normal rhesus monkey erythrocytes and erythrocytes infected by P. knowlesi were labelled with galactose oxidase (EC 1.1.3.9) and tritiated sodium borohydride. The glycoproteins of normal erythrocytes were not labelled unless the cells were pretreated with neuraminidase, when peaks of activity with apparent molecular weights of 170 000, 126 000, 90 000, 50 000, and 35 000 were observed. Schizont-infected erythrocytes showed an absence of glycoprotein labelling even after neuraminidase treatment. The results indicate that there is an alteration in the glycoproteins of schizont-infected erythrocytes, which may contribute to the increased permeability and the immunological alterations on the surface of these cells.

摘要

正常恒河猴红细胞和感染诺氏疟原虫的红细胞用半乳糖氧化酶(EC 1.1.3.9)和氚化硼氢化钠进行标记。正常红细胞的糖蛋白不会被标记,除非细胞先用神经氨酸酶预处理,此时会观察到表观分子量为170000、126000、90000、50000和35000的活性峰。即使经过神经氨酸酶处理,裂殖体感染的红细胞也未显示糖蛋白标记。结果表明,裂殖体感染的红细胞糖蛋白存在改变,这可能导致这些细胞表面通透性增加和免疫改变。

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