Margni R A, Hajos S E
Immunology. 1973 Aug;25(2):323-32.
The methods for isolation and purification of a guinea-pig serum protein with homocytotropic antibody activity and characteristics of IgE are described. By precipitation in the equivalence zone or immunoadsorption and chromatography on DEAE-cellulose, we isolated an homocytotropic antibody, that was not able to give a precipitin line when it was reacted directly with the antigen. It was capable of sensitizing guinea-pig skin for PCA after a latent period of 24–48 hours but not after 3 hours; it was sensitive to treatment with mercaptoethanol. It had antigenic determinants present in the other guinea-pig immunoglobulins and particular antigenic determinants. All these properties make us believe that this protein belongs to an immunoglobulin different from γ and similar to the reaginic antibody (IgE) described in other species.
本文描述了具有亲同种细胞抗体活性的豚鼠血清蛋白的分离纯化方法及其IgE特性。通过在等价区沉淀或免疫吸附以及DEAE - 纤维素柱层析，我们分离出一种亲同种细胞抗体，该抗体直接与抗原反应时不能形成沉淀线。它能够在24 - 48小时的潜伏期后使豚鼠皮肤对被动皮肤过敏反应（PCA）敏感，但3小时后则不能；它对巯基乙醇处理敏感。它具有其他豚鼠免疫球蛋白中存在的抗原决定簇以及特定的抗原决定簇。所有这些特性使我们相信这种蛋白质属于一种不同于γ球蛋白的免疫球蛋白，并且类似于其他物种中描述的反应素抗体（IgE）。
