铁蛋白偶联抗体的抗原结合能力和亲和力。
Antigen-binding capacity and affinity of ferritin conjugated antibodies.
作者信息
Wernet D, Vogt A
出版信息
Immunology. 1973 Oct;25(4):687-95.
Abstract
Anti-DNP-globulin from hyperimmunized rabbits was conjugated to ferritin by means of the bifunctional reagent, metaxylene diisocyanate. About 37 per cent of the antibody linked to ferritin was still able to combine specifically with the antigen, whereas no precipitating activity could be demonstrated. Data obtained in equilibrium dialysis experiments indicate an unchanged valency and affinity of those immunoferritin conjugates which remained active.
摘要
通过双功能试剂间二甲苯二异氰酸酯，将来自高度免疫兔的抗二硝基苯基球蛋白与铁蛋白结合。与铁蛋白相连的抗体中约37%仍能与抗原特异性结合，然而未显示出沉淀活性。平衡透析实验获得的数据表明，那些仍具活性的免疫铁蛋白结合物的价态和亲和力未发生变化。
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