Hämmerling U, Aoki T, de Harven E, Boyse E A, Old L J
J Exp Med. 1968 Dec 1;128(6):1461-73. doi: 10.1084/jem.128.6.1461.
Hybrid antibody [F(ab')(2)] with dual specificity for mouse gammaG and ferritin was prepared from the corresponding rabbit antisera, providing a precise reagent for locating mouse gammaG on cell surfaces. Viable cells were exposed successively to (a) mouse antibody to a cell surface antigen, (b) the rabbit hybrid antibody, and (c) ferritin, before preparation for electron microscopy. This method of See PDF for Structure. labeling is sensitive and specific and clearly lends itself to the introduction of visual markers other than ferritin. Other advantages are uniformity of labeling, ease of purification of the reagent, and circumvention of the many drawbacks arising from coupling ferritin to antibody chemically.
具有针对小鼠γG和铁蛋白双重特异性的杂交抗体[F(ab')(2)]是从相应的兔抗血清中制备的，为在细胞表面定位小鼠γG提供了一种精确的试剂。在制备用于电子显微镜观察的样品之前，将活细胞依次暴露于(a)针对细胞表面抗原的小鼠抗体、(b)兔杂交抗体和(c)铁蛋白。这种标记方法灵敏且特异，显然适用于引入除铁蛋白之外的视觉标记物。其他优点包括标记的均匀性、试剂纯化的简便性以及避免了因化学方法将铁蛋白与抗体偶联而产生的许多缺点。 见PDF获取结构。
