Leslie R G, Cohen S
Immunology. 1974 Oct;27(4):589-99.
IgG2 from immune guinea-pig sera collected 1-2 weeks after secondary BGG challenge showed considerably enhanced cytophilic activity; the preparations contained aggregates (±10 per cent of the total immunoglobulin) shown by antigen exchange to be immune complexes with a probable composition Ag (fragments) Ab. After removal of aggregates the cytophilic activity of immune 7S IgG2 approximated that of IgG2 from unimmunized animals. Artificial immune complexes from either non-immune or immune 7S IgG2 (aggregated with the F(ab') fragments of rabbit anti-guinea-pig Fab) showed greatly enhanced cytophilic activity, maximal at antigen: antibody equivalence and comparable for both IgG2 preparations. These data are consistent with the view that cytophilic activity for macrophages is a uniform property of the whole guinea-pig IgG2 class. The IgG2 complexes apparently bind to glass-adherent cells and also to a population of non-glass-adherent mononuclear cells, present in peritoneal exudates but not detectable in peripheral blood.
二次接种BGG 1 - 2周后收集的免疫豚鼠血清中的IgG2表现出显著增强的亲细胞活性；制剂中含有聚集体（约占总免疫球蛋白的10%），通过抗原交换显示为免疫复合物，其可能的组成为Ag（片段）Ab。去除聚集体后，免疫7S IgG2的亲细胞活性接近未免疫动物的IgG2。由非免疫或免疫7S IgG2（与兔抗豚鼠Fab的F(ab')片段聚集）形成的人工免疫复合物表现出大大增强的亲细胞活性，在抗原与抗体等价时达到最大值，且两种IgG2制剂相当。这些数据与以下观点一致，即对巨噬细胞的亲细胞活性是整个豚鼠IgG2类的统一特性。IgG2复合物显然与玻璃黏附细胞结合，也与存在于腹腔渗出液中但在外周血中检测不到的一群非玻璃黏附单核细胞结合。
