Van Boxel J A, Rosenstreich D L
J Exp Med. 1974 Apr 1;139(4):1002-12. doi: 10.1084/jem.139.4.1002.
Heat-aggregated guinea pig gamma-globulin was shown to bind to the surface membrane of a subclass of guinea pig T lymphocytes. Cells of this subpopulation were identified as T lymphocytes because these cells did not stain for surface Ig (a B-cell marker) but did form spontaneous E-rosettes with rabbit erythrocytes (a T-cell marker). A strikingly high proportion of such aggregate-binding (Agg(+)), E-rosette-forming (E-rosette(+)), but surface Ig-negative (Ig(-)) cells were found in an inflammatory exudate. Thus purified peritoneal exudate lymphocytes (PELs) are known to consist of over 90% T cells, and 59% of these cells bound aggregates. 10% of these Agg+ Ig- E-rosette+ cells were found in draining lymph node cell populations and none in thymus cell populations. The high frequency amongst PELs suggested that these Aggregate+ Ig- E-rosette+ cells might be activated T cells as these are known to occur in high proportion in PEL populations. Confirmatory evidence for this postulate was provided by the striking increase (from 10% to 46%) of Ig- E-rosette+ cells that bound aggregates when lymph node cells were activated by antigen stimulation in vitro.
热聚集的豚鼠γ球蛋白被证明能与豚鼠T淋巴细胞亚类的表面膜结合。该亚群细胞被鉴定为T淋巴细胞，因为这些细胞表面免疫球蛋白（一种B细胞标志物）不着色，但能与兔红细胞（一种T细胞标志物）形成自发E花环。在炎性渗出物中发现了比例极高的此类能结合聚集物（Agg(+)）、形成E花环（E花环(+)）但表面免疫球蛋白阴性（Ig(-)）的细胞。已知纯化的腹腔渗出淋巴细胞（PEL）中T细胞占比超过90%，其中59%的细胞能结合聚集物。在引流淋巴结细胞群体中发现10%的这些Agg+ Ig- E花环+细胞，而胸腺细胞群体中未发现。PEL中此类细胞的高频率表明，这些Aggregate+ Ig- E花环+细胞可能是活化的T细胞，因为已知它们在PEL群体中占比很高。当淋巴结细胞在体外被抗原刺激激活时，能结合聚集物的Ig- E花环+细胞显著增加（从10%增至46%），为这一假设提供了确证。
