Krueger R G
J Virol. 1975 Nov;16(5):1137-45. doi: 10.1128/JVI.16.5.1137-1145.1975.
The host range of the C particle produced by FLOPC-1 myeloma cells, FLOPC-1 murine myeloma-associated virus (FL-MuMAV), was assessed in terms of its ability to productively infect and/or induce new viral antigens in a variety of different cell lines. Production of C particle-like structures by cells exposed to FL-MuMAV) was determined by incorporation of [3H]uridine into particles with a density of 1.16 g/ml and/or measurement of RNA-dependent DNA polymerase activity in concentrated culture medium. to FL-MuMAV was capable of infecting NIH/3T3, normal rat kidney (NRK) cell, BALB/c 3T3, and the A31 clone of BALB/3T3 cells but not rabbit cell line, SIRC. Thus, it is an N, B-tropic murine virus as replication in NRK cells has been shown not to delineate a group of murine viruses with a separate host range (M. M. Lieber, C. J. Sherr, and G. J. Todero, 1974). Further neoantigens, reactive with anti-FL-MuMAV serum, were detected on infected cells. Production of the MuMAV-like particle and MuMAV-associated cell antigens in infected NIH/3T3 and NRK cells persisted for three subcultures. The limited production could not be explained by the lack of an RNA-dependent DNA polymerase or high-molecular-weight RNA as the particles possessed both of these properties. The particles produced by infected NIH/3T3 or NRK cells were antigenically and physicochemically similar to FL-MuMAV and not K-MuLV. The MuMAV-like particles produced by infected NIH/3T3 were capable of limited replication in NIH/3T3 and and BALB/3T3 cells, whereas NRK-MuMAV replicated for a limited period in NIH/3T3, NRK, and SIRC cells; i.e., they had a different host range than FL-MuMAV. The particles produced by infected BALB/3T3 and A31 cells had the same host range as FL-MuMAV. In certain situations, isotopically labeled particles with a density of 1.16 g/ml were produced which appeared to lack RNA-dependent DNA polymerase.
对由FLOPC - 1骨髓瘤细胞产生的C颗粒，即FLOPC - 1鼠骨髓瘤相关病毒（FL - MuMAV）的宿主范围，根据其在多种不同细胞系中有效感染和/或诱导新病毒抗原的能力进行了评估。通过将[3H]尿苷掺入密度为1.16 g/ml的颗粒中，和/或通过测量浓缩培养基中的RNA依赖性DNA聚合酶活性，来确定暴露于FL - MuMAV的细胞产生C颗粒样结构的情况。FL - MuMAV能够感染NIH/3T3、正常大鼠肾（NRK）细胞、BALB/c 3T3以及BALB/3T3细胞的A31克隆，但不能感染兔细胞系SIRC。因此，它是一种N、B嗜性鼠病毒，因为已证明在NRK细胞中的复制并不能界定出具有单独宿主范围的一组鼠病毒（M. M. Lieber、C. J. Sherr和G. J. Todero，1974年）。在感染细胞上还检测到了与抗FL - MuMAV血清反应的更多新抗原。在感染的NIH/3T3和NRK细胞中，MuMAV样颗粒和MuMAV相关细胞抗原的产生持续了三次传代培养。产量有限不能用缺乏RNA依赖性DNA聚合酶或高分子量RNA来解释，因为这些颗粒同时具备这两种特性。感染的NIH/3T3或NRK细胞产生的颗粒在抗原性和物理化学性质上与FL - MuMAV相似，而与K - MuLV不同。感染的NIH/3T3产生的MuMAV样颗粒能够在NIH/3T3和BALB/3T3细胞中进行有限复制，而NRK - MuMAV在NIH/3T3、NRK和SIRC细胞中能有限期复制；也就是说，它们的宿主范围与FL - MuMAV不同。感染的BALB/3T3和A31细胞产生的颗粒与FL - MuMAV具有相同的宿主范围。在某些情况下，会产生密度为1.16 g/ml的同位素标记颗粒，这些颗粒似乎缺乏RNA依赖性DNA聚合酶。
