Thomason B M, Hebert G A
Appl Microbiol. 1974 May;27(5):862-9. doi: 10.1128/am.27.5.862-869.1974.
Fluorescent antibody (FA) reagents for Salmonella produced by Difco, Sylvana, and Clinical Sciences, Inc., were evaluated for physicochemical and performance characteristics. The Difco panvalent (A through 064) and the Difco polyvalent (A through S) were similar in physicochemical characteristics. They had less than 60% gamma globulin with 3% albumin and had fluorescein to protein (F/P) ratios of less than 10. The Sylvana conjugate had 81% gamma globulin with less than 1% albumin. Its F/P was 33.9. The Clinical Sciences reagent contained 75% unlabeled albumin as packaged in the Fluoro-kit. Analysis of the original conjugate showed 86.5% gamma globulin with only 0.5% albumin. The (F/P) was 32.8. The performance characteristics were determined by using a variety of Enterobacteriaceae and food and feed samples. All conjugates stained the homologous Salmonella strains. The majority of cross-reactions were limited primarily to the Arizona, Citrobacter, and Escherichia coli groups. The Difco panvalent was more reactive with heterologous organisms. It stained 89% of the Arizona compared with 42% stained by the Difco polyvalent (A through S) and 39% stained by the Sylvana and Clinical Sciences reagents. We found 90% agreement between FA and culture when the Difco polyvalent was used to examine food and feed samples and 94% agreement when the Clinical Sciences Fluoro-kit was used on another group of samples.
对迪夫科公司（Difco）、西尔瓦纳公司（Sylvana）和临床科学公司（Clinical Sciences, Inc.）生产的沙门氏菌荧光抗体（FA）试剂进行了物理化学和性能特性评估。迪夫科的多价（A至064）和迪夫科的多价（A至S）在物理化学特性上相似。它们的γ球蛋白含量低于60%，白蛋白含量为3%，荧光素与蛋白质（F/P）的比率低于10。西尔瓦纳偶联物的γ球蛋白含量为81%，白蛋白含量低于1%。其F/P为33.9。临床科学试剂在荧光试剂盒包装中含有75%未标记的白蛋白。对原始偶联物的分析显示γ球蛋白含量为86.5%，白蛋白仅为0.5%。（F/P）为32.8。通过使用多种肠杆菌科细菌以及食品和饲料样本确定性能特性。所有偶联物均能对同源沙门氏菌菌株染色。大多数交叉反应主要局限于亚利桑那菌属、柠檬酸杆菌属和大肠杆菌属。迪夫科多价试剂与异源生物体的反应性更强。它对89%的亚利桑那菌属进行了染色，相比之下，迪夫科多价（A至S）试剂染色的比例为42%，西尔瓦纳和临床科学试剂染色的比例为39%。当使用迪夫科多价试剂检测食品和饲料样本时，我们发现荧光抗体检测法（FA）与培养法之间的一致性为90%；当使用临床科学荧光试剂盒检测另一组样本时，一致性为94%。
