用于检测食品和饲料中沙门氏菌的快速直接荧光抗体法。
Rapid, direct fluorescent-antibody method for the detection of salmonellae in food and feeds.
作者信息
Insalata N F, Mahnke C W, Dunlap W G
出版信息
Appl Microbiol. 1972 Oct;24(4):645-9. doi: 10.1128/am.24.4.645-649.1972.
Abstract
An improved immunofluorescent-antibody (FA) method for the detection of salmonellae in foods and feeds was developed. This FA method combines a rapid cultural phase and a serological phase that allow for propagation of salmonellae in a minimum time, employing the industrial 8-hr work day as a guide. Two hundred fifty naturally contaminated human food and animal feed samples, representing 647 trials, were tested by the FA method. A total of 18 different food and feed samples was used. The method used by the Association of Official Analytical Chemists (AOAC) for the detection of salmonellae was the control method. The percent agreement when comparing the FA slide method to the AOAC method ranged from 87.1 to 95.3%, depending upon the conjugated antisera used in comparative studies.
摘要
开发了一种改进的免疫荧光抗体（FA）方法，用于检测食品和饲料中的沙门氏菌。这种FA方法结合了快速培养阶段和血清学阶段，以工业8小时工作日为指导，能够在最短时间内使沙门氏菌繁殖。通过FA方法对代表647次试验的250份天然污染的人类食品和动物饲料样本进行了检测。总共使用了18种不同的食品和饲料样本。官方分析化学家协会（AOAC）用于检测沙门氏菌的方法作为对照方法。将FA玻片法与AOAC法进行比较时，一致性百分比在87.1%至95.3%之间，这取决于比较研究中使用的共轭抗血清。
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