Mozes E, Schwartz M, Sela M
J Exp Med. 1974 Aug 1;140(2):349-55. doi: 10.1084/jem.140.2.349.
Five inbred mouse strains which represent high and low responders to the random synthetic polypeptide poly(LTyr,LGlu)-polyDLAla--polyLLys, designated (T, G)-A--L, to which the immune response is controlled by an H-2-linked gene, were immunized with three ordered tetrapeptides composed of tyrosine and glutamic acid attached either to multichain poly-DL-alanine or to polyproline. Only one of the three antigenic determinants, namely tyrosyl-tyrosyl-glytamyl-glutamic acid (T-T-G-G), resembled the random peptide (T, G) in the pattern of immune responses elicited against it, and in the cross-reactivity of the specific antibodies with (T, G)-A--L. The immune response pattern to the other two ordered tetrapeptides, T-G-T-G and G-T-T-G, was different from that obtained with (T, G)-A--L, and no cross-reactivity was detected between the antibodies provoked with these peptides and (T, G)-A--L. Thus, it is suggested that T-T-G-G is a major determinant in the random (T, G)-A--L.
五种近交系小鼠对随机合成多肽聚(L-酪氨酸,L-谷氨酸)-聚-DL-丙氨酸-聚-L-赖氨酸,即(T,G)-A-L表现出高反应性和低反应性,其免疫反应由一个与H-2连锁的基因控制。用三种由酪氨酸和谷氨酸组成的有序四肽对其进行免疫,这些四肽连接在多链聚-DL-丙氨酸或聚脯氨酸上。三种抗原决定簇中只有一种,即酪氨酰-酪氨酰-谷氨酰-谷氨酸(T-T-G-G),在引发的免疫反应模式以及特异性抗体与(T,G)-A-L的交叉反应性方面与随机肽(T,G)相似。对另外两种有序四肽T-G-T-G和G-T-T-G的免疫反应模式与用(T,G)-A-L获得的不同,并且在用这些肽激发的抗体与(T,G)-A-L之间未检测到交叉反应性。因此,有人提出T-T-G-G是随机(T,G)-A-L中的主要决定簇。