乙肝抗原：通过亲和层析揭示的与人类血清蛋白相关的抗原位点。
Hepatitis B antigen: antigenic sites related to human serum proteins revealed by affinity chromatography.
作者信息
Neurath A R, Prince A M, Lippin A
出版信息
Proc Natl Acad Sci U S A. 1974 Jul;71(7):2663-7. doi: 10.1073/pnas.71.7.2663.
Abstract
Hepatitis B antigen-associated particles, isolated from sera of antigen carriers, were submitted to affinity chromatography on columns of insolubilized antibodies to normal human plasma. The particles adsorbed to the immunosorbent at pH 7.2 and were subsequently eluted at pH 2.2. Exposure of the particles to 8 M urea, 5 M KI, pH 2.2, detergents, organic solvents, or proteolytic enzymes failed to prevent their subsequent adsorption to the immunosorbent. This suggests that antigenic determinants related to human plasma proteins are constituent components of hepatitis B antigen-associated particles. These determinants are distinct from the group-specific (a) and subtype-specific (d or y) sites of the hepatitis B antigen and appear to be related to antigenic specificities on prealbumin, albumin, apolipoproteins C and D, and the gamma-chain of immunoglobulin G.
摘要
从抗原携带者血清中分离出的乙型肝炎抗原相关颗粒，被用于在固定化抗正常人血浆抗体柱上进行亲和层析。这些颗粒在pH 7.2时吸附到免疫吸附剂上，随后在pH 2.2时被洗脱。将这些颗粒暴露于8M尿素、5M碘化钾、pH 2.2、去污剂、有机溶剂或蛋白水解酶中，均未能阻止它们随后吸附到免疫吸附剂上。这表明与人类血浆蛋白相关的抗原决定簇是乙型肝炎抗原相关颗粒的组成成分。这些决定簇不同于乙型肝炎抗原的群特异性（a）和亚型特异性（d或y）位点，似乎与前白蛋白、白蛋白、载脂蛋白C和D以及免疫球蛋白G的γ链上的抗原特异性有关。
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