乙肝表面抗原（HBsAg）中与人类血清蛋白相关的抗原位点：从正常人血清中分离出一种与抗白蛋白反应的高分子量糖蛋白

Antigenic sites related to human serum proteins in HBsAg: isolation from normal human serum of a high-molecular-weight glycoprotein reacting with antialbumin.

作者信息

Neurath A R, Strick N, Huang C Y, Prince A M

出版信息

J Med Virol. 1978;2(3):231-45. doi: 10.1002/jmv.1890020307.

DOI:10.1002/jmv.1890020307

Abstract

A novel minor constituent was isolated from normal human serum by affinity chromatography on columns of insolubilized concanavalin A and antibodies to albumin, followed by rate zonal centrifugation. This component has the following properties: a sedimentation coefficient of approximately 31; a diameter of 14--22 nm, and a buoyant density of 1.302 gm/cu cm. It contains about 1% neutral sugars and is electrophoretically heterogeneous, since two populations of particles with isoelectric points of pH 4.95 and 5.75 were separated by isoelectric focusing. It contains a single major glycopeptide with an apparent molecular weight of 72,000 daltons. Its amino acid composition is distinct from that of albumin. It elicited the formation of antibodies that also reacted with HBsAg.

摘要

通过在固定化伴刀豆球蛋白A和抗白蛋白抗体柱上进行亲和层析，随后进行速率区带离心，从正常人血清中分离出一种新型次要成分。该成分具有以下特性：沉降系数约为31；直径为14 - 22纳米，浮力密度为1.302克/立方厘米。它含有约1%的中性糖，且在电泳上具有异质性，因为通过等电聚焦分离出了两个等电点分别为pH 4.95和5.75的粒子群体。它含有一种表观分子量为72,000道尔顿的单一主要糖肽。其氨基酸组成与白蛋白不同。它能引发抗体的形成，这些抗体也与乙肝表面抗原发生反应。