乙肝表面抗原大分子量多肽与人类血清白蛋白的关系。
Relationship of large hepatitis B surface antigen polypeptide to human serum albumin.
作者信息
Shih J W, Tan P L, Gerin J L
出版信息
Infect Immun. 1980 May;28(2):459-63. doi: 10.1128/iai.28.2.459-463.1980.
A significant proportion (20 to 40%) of highly purified 22-nm hepatitis B surface antigen (HBsAg) particles contain human serum albumin (HSA) as demonstrated by specific precipitation of radioiodinated particles by anti-HSA. Preparations of the isolated major HBsAg polypeptides (P-1, P-2, and P-6) were iodinated and analyzed by radiommunoprecipitation for reactivity with rabbit antisera to human plasma proteins. Only the P-6 fraction (molecular weight, 68,000) was precipitated and only by anti-HSA; specific precipitation was observed with guinea pig antisera to P-6 and native HBsAg and goat or rabbit antisera to HSA. Coprecipitation of P-6 with antiserum to HBsAg and with anti-HSA, compared to precipitation with each antiserum alone, indicated that the HBsAg and HSA determinants were on separate molecules. The P-6 polypeptide may represent a precursor protein of the hepatitis B virion.
通过抗人血清白蛋白（HSA）对放射性碘化颗粒的特异性沉淀证明，高度纯化的22纳米乙型肝炎表面抗原（HBsAg）颗粒中有很大一部分（20%至40%）含有HSA。对分离出的主要HBsAg多肽（P-1、P-2和P-6）制剂进行碘化，并通过放射免疫沉淀分析其与兔抗人血浆蛋白血清的反应性。只有P-6组分（分子量为68,000）被沉淀，且仅被抗HSA沉淀；用豚鼠抗P-6和天然HBsAg血清以及山羊或兔抗HSA血清观察到特异性沉淀。与单独使用每种抗血清的沉淀相比，P-6与抗HBsAg血清和抗HSA血清的共沉淀表明，HBsAg和HSA决定簇位于不同的分子上。P-6多肽可能代表乙型肝炎病毒粒子的前体蛋白。
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