使用灵敏的微量浊度计对抗原和沉淀抗体进行测定。
The use of a sensitive micronephelometer in the estimation of antigens and precipitating antibodies.
作者信息
Davies G E
出版信息
Immunology. 1971 May;20(5):779-87.
A very sensitive micronephelometer has been used to measure the light-scattering of dilute mixtures of antigen solutions and precipitating antibodies. At equivalent ratios of antigen and antibody, the degree of light scattering is proportional to the protein content of the complex thus allowing estimates of antibody protein to be made. In the region of antibody excess, light scattering is proportional to the concentration of antigen thus allowing a calibration curve to be drawn which may be used in the estimation of antigen. The technique is quick and simple and the results are not markedly affected by moderate changes in pH, salt concentration, temperature or period of incubation.
一种非常灵敏的微浊度计已被用于测量抗原溶液与沉淀抗体的稀释混合物的光散射。在抗原与抗体的当量比下，光散射程度与复合物的蛋白质含量成正比，从而可以对抗体蛋白质进行估算。在抗体过量的区域，光散射与抗原浓度成正比，从而可以绘制校准曲线，用于抗原的估算。该技术快速简便，pH值、盐浓度、温度或孵育时间的适度变化对结果没有明显影响。
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