Vincent W F, Harris E W, Yaverbaum S
Immunology. 1970 Feb;18(2):143-7.
The development of turbidity resulting from the precipitation between bovine serum albumin (BSA) and anti-BSA serum was studied in the spectrophotometer. Data obtained with the turbidimetric method correlated well with those obtained using the classical tube precipitin determination. The equivalence ratio and the concentration of precipitating antibody were determined turbidimetrically by a comparison of the data from both methods. Antibody concentration can be determined with this method in about 1 hour as compared with the 3 or more days required by the tube test. In addition, this technique requires substantially less reagent than other methods.
利用分光光度计研究了牛血清白蛋白（BSA）与抗BSA血清之间沉淀所导致的浊度变化。用比浊法获得的数据与经典试管沉淀试验所得数据高度相关。通过比较两种方法的数据，用比浊法测定了等价比和沉淀抗体的浓度。与试管试验需要3天或更长时间相比，用该方法大约1小时就能测定抗体浓度。此外，该技术所需试剂比其他方法少得多。
