Loni M C, Green M
Proc Natl Acad Sci U S A. 1974 Sep;71(9):3418-22. doi: 10.1073/pnas.71.9.3418.
Cytological preparations of interphase nuclei and chromosomes from mouse 3T6 cells prepared at various times after infection with the murine sarcomaleukemia virus complex were hybridized with the [(3)H]DNA product of the viral RNA-directed DNA polymerase. While uninfected nuclei had an average of 4 autoradiographic grains, infected nuclei had 30 grains at 5 hr after infection and 63-65 grains at 11 and 25 hr. Virus-specific grains were localized in the chromocenters of interphase nuclei and were found also in the centromeric heterochromatin region of metaphase chromosomes. These findings provide evidence that the viral RNA-directed DNA polymerase functions to synthesize virus-specific DNA early after infection and that newly synthesized viral DNA rapidly becomes associated with or integrated into specific intranuclear sites.
用鼠肉瘤白血病病毒复合体感染小鼠3T6细胞，在感染后不同时间制备的间期核和染色体的细胞学标本，与病毒RNA指导的DNA聚合酶的[³H]DNA产物进行杂交。未感染的核平均有4个放射自显影颗粒，感染的核在感染后5小时有30个颗粒，在11小时和25小时有63 - 65个颗粒。病毒特异性颗粒定位于间期核的染色中心，也存在于中期染色体的着丝粒异染色质区域。这些发现证明，病毒RNA指导的DNA聚合酶在感染后早期发挥作用，合成病毒特异性DNA，并且新合成的病毒DNA迅速与特定的核内位点结合或整合到其中。
