Fv-1基因产物对感染鼠白血病病毒的细胞中前病毒DNA形成和整合的影响。
Effect of Fv-1 gene product on proviral DNA formation and integration in cells infected with murine leukemia viruses.
作者信息
Jolicoeur P, Baltimore D
出版信息
Proc Natl Acad Sci U S A. 1976 Jul;73(7):2236-40. doi: 10.1073/pnas.73.7.2236.
Abstract
The amounts of unintegrated murine leukemia virus-specific DNA detected by molecular hybridization in extracts of Fv-ln/n (strains NIH/3T3, SIM) or Fv-lb/b (strains JLS-V9, SIM.R) mouse cells after infection with N- or B-tropic viruses were found to be the same in both permissive and resistant cells. Therefore, formation of DNA products from the viral RNA template does not appear to be grossly affected by the Fv-l gene product. Integration of virus-specific DNA into chromosomal cellular DNA was assayed by hybridization of radioactive complementary DNA to DNA from infected cells. With either NIH/3T3 or SIM.R cells infected with N- or B-tropic viruses, integration of proviral DNA could be detected in permissive cells but not in nonpermissive cells. The Fv-l gene product therefore appears to prevent integration of proviral DNA.
摘要
在用N-嗜性或B-嗜性病毒感染后，通过分子杂交在Fv-1n/n（NIH/3T3、SIM株）或Fv-1b/b（JLS-V9、SIM.R株）小鼠细胞提取物中检测到的未整合的鼠白血病病毒特异性DNA的量，在允许细胞和抗性细胞中是相同的。因此，从病毒RNA模板形成DNA产物似乎没有受到Fv-1基因产物的严重影响。通过将放射性互补DNA与感染细胞的DNA杂交来检测病毒特异性DNA整合到染色体细胞DNA中。在用N-嗜性或B-嗜性病毒感染的NIH/3T3或SIM.R细胞中，在允许细胞中可以检测到前病毒DNA的整合，但在非允许细胞中则检测不到。因此，Fv-1基因产物似乎可以阻止前病毒DNA的整合。
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