Hatanaka M, Kakefuda T, Gilden R V, Callan E A
Proc Natl Acad Sci U S A. 1971 Aug;68(8):1844-7. doi: 10.1073/pnas.68.8.1844.
Infection of mouse embryo cells with two strains of murine sarcoma virus and one strain of murine leukemia virus was followed rapidly by synthesis of DNA in the cytoplasm. Persistently infected cells have not shown such synthesis, and ultraviolet-irradiated virus did not induce DNA synthesis. This new DNA presumably represents an intermediate in the virus replication cycle specified by the virion DNA polymerase(s). Failure to observe cytoplasmic DNA synthesis in persistently infected cells suggests, in keeping with the results of inhibitor experiments, that the new "viral" DNA becomes associated with cellular DNA in some form of stable interaction.
用两株鼠肉瘤病毒和一株鼠白血病病毒感染小鼠胚胎细胞后，细胞质中很快就会合成DNA。持续感染的细胞未出现这种合成现象，紫外线照射的病毒也未诱导DNA合成。这种新的DNA大概代表了由病毒粒子DNA聚合酶指定的病毒复制周期中的一个中间体。与抑制剂实验结果一致，在持续感染的细胞中未观察到细胞质DNA合成，这表明新的“病毒”DNA以某种稳定相互作用的形式与细胞DNA结合。
