兔珠蛋白互补DNA合成的RNA的核苷酸序列分析
Nucleotide sequence analysis of RNA synthesized from rabbit globin complementary DNA.
作者信息
Poon R, Paddock G V, Heindell H, Whitcome P, Salser W, Kacian D, Bank A, Gambino R, Ramirez F
出版信息
Proc Natl Acad Sci U S A. 1974 Sep;71(9):3502-6. doi: 10.1073/pnas.71.9.3502.
Rabbit globin complementary DNA made with RNA-dependent DNA polymerase (reverse transcriptase) was used as template for in vitro synthesis of (32)P-labeled RNA. The sequences of the nucleotides in most of the fragments resulting from combined ribonuclease T(1) and alkaline phosphatase digestion have been determined. Several fragments were long enough to fit uniquely with the alpha or beta globin amino-acid sequences. These data demonstrate that the cDNA was copied from globin mRNA and contained no detectable contaminants.
用依赖RNA的DNA聚合酶（逆转录酶）制备的兔珠蛋白互补DNA作为体外合成（32）P标记RNA的模板。已经确定了核糖核酸酶T（1）和碱性磷酸酶联合消化产生的大多数片段中核苷酸的序列。几个片段足够长，可以与α或β珠蛋白氨基酸序列唯一匹配。这些数据表明，该cDNA是从珠蛋白mRNA复制而来的，并且没有可检测到的污染物。
Zotero 插件