Capanna S L, Kong Y M
Immunology. 1974 Oct;27(4):647-53.
Poly A:U, a complex of synthetic polyadenylic—polyuridylic acids, prevented tolerance induction to bovine gamma—globulin (BGG) in mice when administered 0-12 hours after a tolerogenic dose (500 μg) of BGG, as shown by haemagglutinin production and immune elimination of labelled antigen after challenge with 300 μg of aggregated BGG. Administration of poly A:U 3-6 hours after tolerogen appeared to be the most effective. It was found further that two doses of poly A:U given at 3 and 6 or 3 and 12 hours, or a double dose of 600 μg given at 3 hours after tolerogen did not increase its effectiveness beyond that of a single dose of 300 μg given at 3 hours. The finding that poly A:U, which may affect thymus-derived (T) cell function, prevented tolerance induction to a T cell-dependent antigen suggests that the action of poly A:U is on the T cell. For comparison, endotoxin, thought to affect bone marrow-derived (B) cell function, was included and it interfered with tolerance induction when given 0-72 hours after soluble BGG. This interval is in marked contrast to poly A:U which is effective at 0-12 hours after tolerogen and probably reflects a difference in mechanism of the two agents.
尿苷酸（Poly A:U），一种合成的聚腺苷酸 - 聚尿苷酸复合物，在给予小鼠致耐受性剂量（500μg）的牛γ球蛋白（BGG）后0 - 12小时给药时，可防止小鼠对BGG产生耐受性，这通过血凝素产生以及在用300μg聚集BGG攻击后标记抗原的免疫清除得以证明。在给予致耐受原后3 - 6小时给予Poly A:U似乎最为有效。进一步发现，在致耐受原后3小时和6小时或3小时和12小时给予两剂Poly A:U，或者在致耐受原后3小时给予600μg的双倍剂量，其效果并不比在3小时给予300μg单剂量的效果更好。Poly A:U可能影响胸腺来源（T）细胞功能，它能防止对T细胞依赖性抗原产生耐受性，这一发现表明Poly A:U的作用靶点是T细胞。为作比较，纳入了被认为影响骨髓来源（B）细胞功能的内毒素，当在可溶性BGG后0 - 72小时给予时，它会干扰耐受性诱导。这个时间间隔与Poly A:U形成鲜明对比，Poly A:U在致耐受原后0 - 12小时有效，这可能反映了这两种物质作用机制的差异。
