Snippe H, van Eyk R V
Immunology. 1974 Nov;27(5):771-9.
The target cell of stimulation of primed spleen cells by hapten—carrier complexes was studied. The antigens DNP—bovine serum albumin (DNP—BSA), which gives only 2,4-dinitrophenyl (DNP) specific responses and DNP—mouse immunoglobulin (DNP—MIg), which probably reveals DNP as well as carrier specificity (new antigenic determinant) were used. Educated T cells could be stimulated with the antigens used for activation. A similar experiment with educated B cells, however, gave no indication that these B cells could be stimulated with antigen in the absence of T cells. Cortisone treatment of primed mice yielded spleen cells which had a higher activity than spleen cells from unprimed, cortisone-treated mice. This also points to stimulation of T cells by antigen. Treatment of primed spleen cells with anti-thymocyte serum (ATS) and complement (C) abolished stimulation by phytohaemagglutinin (PHA) completely, by lipopolysaccharide (LPS) slightly, while the antigen-specific activity was 90 per cent reduced. This indicates a mainly T cell-specific stimulation by the antigen. A corresponding experiment with anti-plasma cell serum (APCS) and C revealed a complete reduction of LPS activity and a small impairment of the PHA and conavalin A (Con A) activity. However, the antigen-specific activity was reduced by one-third to a half for the different antigens. This is an indication for a specific B-cell stimulation by the antigen, although it is on a lower level than the T-cell stimulation. The role of the hapten in the T-cell stimulation is discussed.
研究了半抗原-载体复合物对致敏脾细胞的刺激靶细胞。使用了仅产生2,4-二硝基苯基（DNP）特异性反应的抗原二硝基苯基-牛血清白蛋白（DNP-BSA），以及可能揭示DNP和载体特异性（新抗原决定簇）的二硝基苯基-小鼠免疫球蛋白（DNP-MIg）。受过诱导的T细胞可用作激活的抗原进行刺激。然而，对受过诱导的B细胞进行的类似实验并未表明这些B细胞在没有T细胞的情况下能被抗原刺激。用可的松处理致敏小鼠产生的脾细胞比未致敏、经可的松处理的小鼠的脾细胞活性更高。这也表明抗原对T细胞有刺激作用。用抗胸腺细胞血清（ATS）和补体（C）处理致敏脾细胞，可完全消除植物血凝素（PHA）的刺激，轻微降低脂多糖（LPS）的刺激，而抗原特异性活性降低90%。这表明抗原主要对T细胞有特异性刺激。用抗浆细胞血清（APCS）和C进行的相应实验表明，LPS活性完全降低，PHA和刀豆球蛋白A（Con A）活性略有受损。然而，不同抗原的抗原特异性活性降低了三分之一到二分之一。这表明抗原对B细胞有特异性刺激作用，尽管其水平低于对T细胞的刺激。文中讨论了半抗原在T细胞刺激中的作用。
