Kraaijeveld C A, Benaissa-Trouw B J, Harmsen M, Snippe H
Arch Virol. 1986;91(1-2):83-92. doi: 10.1007/BF01316730.
This paper describes the minimal requirements to protect mice adoptively against challenge with virulent Semliki Forest virus (SFV). Early immune serum, from donor mice infected with an avirulent strain of SFV, contained mainly neutralizing IgM immunoglobulins. More of these antibodies (1.80 PND50 vs 0.06 PND50) were needed to protect recipient mice against intraperitoneal challenge (10 LD50 of SFV) than against subcutaneous challenge (7 LD50). Adoptive transfer experiments indicated that a minimum of 3 X 10(7) six-day immune spleen cells were also able to protect recipient mice against intraperitoneal challenge with 10 LD50 of SFV. Treatment of these donor cells with cytotoxic antisera and complement revealed both T- and B-lymphocytes were required for optimum adoptive immunity. Surviving recipients of either immune serum or immune spleen cells developed significantly less neutralizing antibodies than control mice. The lower antibody titres in protected mice might be related to either immune serum or immune spleen cell mediated restriction of virus replication; meaning a reduced antigenic stimulus in these mice compared to control mice.
本文描述了通过过继免疫保护小鼠抵御强毒塞姆利基森林病毒（SFV）攻击的最低要求。来自感染无毒力SFV毒株的供体小鼠的早期免疫血清主要含有中和性IgM免疫球蛋白。相较于皮下攻击（7 LD50的SFV），保护受体小鼠抵御腹腔内攻击（10 LD50的SFV）需要更多此类抗体（1.80 PND50对0.06 PND50）。过继转移实验表明，最少3×10⁷个六日龄免疫脾细胞也能够保护受体小鼠抵御10 LD50的SFV腹腔内攻击。用细胞毒性抗血清和补体处理这些供体细胞表明，最佳过继免疫需要T淋巴细胞和B淋巴细胞。免疫血清或免疫脾细胞的存活受体产生的中和抗体明显少于对照小鼠。受保护小鼠中较低的抗体滴度可能与免疫血清或免疫脾细胞介导的病毒复制受限有关；这意味着与对照小鼠相比，这些小鼠的抗原刺激减少。
