Snippe H, Willems P J, Graven W G, Kamp E
Immunology. 1975 May;28(5):897-907.
Delayed hypersensitivity (DH) in the mouse was studied with complexes of dinitrophenyl (DHP) as hapten and bovine serum albumin (BSA), mouse immunoglobulin (MIg) and polyvinylpyrrolidone (PVP) as carrier. Priming with BSA induced strong DH against this carrier, but DN of decreasing strength against complexes with increasing DNP:carrier ratio. Priming with DNP-BSA complexes never resulted in a DH against BSA or DNP 3-minusBSA. Injections of DNP 16-minusBSA and DNP 28-minusBSA induced positive DH which increased with the hapten:carrier ratio of the eliciting antigen. The complexes with an isologous carrier DNP 48-minusMIg or DNP 90-minusMIg induced positive reactions against both complexes but not against the weakly substituted DNP 11-minusMIg. The latter only primed for itself. The importance of the DNP groups as determinant in these DH reactions is stressed by the cross-reactions between DNP-BSA and DNP-MIg complexes and by the induction by DNP 16-minusPVP of positive DH against DNP 28-minusBSA. Cyclophosphamide (Cy) treatment before priming with complexes induced enhanced DH against complexes with sufficient hapten:carrier ratio. Priming with carrier under Cy treatment induced no DH against complexes. All these results indicate that carrier determinants are not involved in the DH against complexes. After priming wtih complexes with a low hapten:carrier ratio the DH is directed against new antigenic determinants (NAD) groups. After priming with complexes with high ratios DH is directed against DNP groups. With adoptive local transfer of spleen cells of primed animals and pretreatment of these cells with anti-thymocyte serum or anti-plasma cell serum and complement it was possible to demonstrate that the T cell was responsible for the DH reactions. The involvement of different determinant groups on the hapten-carrier complexes in immune reactions is discussed.
以二硝基苯基（DHP）作为半抗原，牛血清白蛋白（BSA）、小鼠免疫球蛋白（MIg）和聚乙烯吡咯烷酮（PVP）作为载体形成的复合物，对小鼠迟发型超敏反应（DH）进行了研究。用BSA进行初次免疫可诱导出针对该载体的强烈DH反应，但随着二硝基苯基（DNP）与载体比例增加，针对复合物的DH反应强度逐渐减弱。用DNP - BSA复合物进行初次免疫从未导致针对BSA或DNP减去BSA的DH反应。注射DNP 16减去BSA和DNP 28减去BSA可诱导出阳性DH反应，且该反应随引发抗原的半抗原与载体比例增加而增强。与同种载体DNP 48减去MIg或DNP 90减去MIg形成的复合物可诱导针对两种复合物的阳性反应，但不针对弱取代的DNP 11减去MIg。后者仅对自身进行初次免疫。DNP - BSA与DNP - MIg复合物之间的交叉反应以及DNP 16减去PVP诱导针对DNP 28减去BSA的阳性DH反应，强调了DNP基团在这些DH反应中作为决定簇的重要性。在用复合物进行初次免疫前用环磷酰胺（Cy）处理，可增强针对具有足够半抗原与载体比例的复合物的DH反应。在Cy处理下用载体进行初次免疫不会诱导针对复合物的DH反应。所有这些结果表明，载体决定簇不参与针对复合物的DH反应。在用低半抗原与载体比例的复合物进行初次免疫后，DH反应针对新的抗原决定簇（NAD）基团。在用高比例复合物进行初次免疫后，DH反应针对DNP基团。通过对经初次免疫动物的脾细胞进行过继性局部转移，并对这些细胞用抗胸腺细胞血清或抗浆细胞血清及补体进行预处理，有可能证明T细胞负责DH反应。文中讨论了半抗原 - 载体复合物上不同决定簇基团在免疫反应中的作用。
