Selgrade M K, Osborn J E
Infect Immun. 1974 Dec;10(6):1383-90. doi: 10.1128/iai.10.6.1383-1390.1974.
The role of macrophages in protecting mice from murine cytomegalovirus (MCMV) was studied in Swiss, CBA/J, and C57BL/6J mice. CBA/J mice were more resistant to virus than were C57BL/6J mice at all ages tested. Prior treatment of adult Swiss mice with 60 mg of silica, a dose selectively toxic to macrophages, increased mortality due to MCMV infection. Transfer of syngeneic adult macrophages to suckling mice significantly increased their resistance to subsequent MCMV infection. Transfer of syngeneic, nonimmune adult lymphocytes to suckling mice also had a lesser but significant protective effect against subsequent MCMV challenge. In vitro infection of adult CBA/J and C57BL/6J macrophages with virulent and attenuated MCMV resulted in productive infection in only a small percentage of cells and recovery of very little virus from the extracellular fluid. Infection of CBA macrophages was no less productive than C57BL/6J nor was infection with virulent virus more productive than with attenuated virus. Histological examination of the livers of MCMV-infected CBA/J and C57BL/6J mice suggested that divergent cellular immune responses to infection might account for differences in susceptibility. It is postulated that the macrophage may facilitate the inductive phase of cellular immunity, one possible explanation for its demonstrated importance in host defenses against MCMV.
在瑞士小鼠、CBA/J小鼠和C57BL/6J小鼠中研究了巨噬细胞在保护小鼠免受鼠巨细胞病毒（MCMV）感染方面的作用。在所有测试年龄中，CBA/J小鼠比C57BL/6J小鼠对病毒更具抵抗力。用60毫克二氧化硅对成年瑞士小鼠进行预处理，该剂量对巨噬细胞具有选择性毒性，会增加MCMV感染导致的死亡率。将同基因成年巨噬细胞转移到乳鼠体内可显著提高它们对随后MCMV感染的抵抗力。将同基因、未免疫的成年淋巴细胞转移到乳鼠体内对随后的MCMV攻击也有较小但显著的保护作用。用强毒株和减毒株MCMV对成年CBA/J和C57BL/6J巨噬细胞进行体外感染，仅在一小部分细胞中导致有效感染，且从细胞外液中回收的病毒极少。CBA巨噬细胞的感染效率并不低于C57BL/6J巨噬细胞，强毒株感染的效率也不高于减毒株感染。对MCMV感染的CBA/J和C57BL/6J小鼠肝脏进行组织学检查表明，对感染的不同细胞免疫反应可能是易感性差异的原因。据推测，巨噬细胞可能促进细胞免疫的诱导阶段，这是其在宿主抵御MCMV防御中显示出重要性的一种可能解释。
