Shanley J D, Pesanti E L
Infect Immun. 1983 Sep;41(3):1352-9. doi: 10.1128/iai.41.3.1352-1359.1983.
Because there have been different conclusions regarding the susceptibility of murine macrophages to murine cytomegalovirus (MCMV) infection and replication, we have undertaken a detailed comparison of MCMV infection of macrophages with that of a permissive cell line, mouse embryo cells. Although both cell lines undergo productive infections with MCMV, there are marked differences in certain aspects of the viral replication which may account for some of the different conclusions regarding the MCMV cycle in macrophages. Although both cell lines produce MCMV after infection, the time course of the infection differed markedly between the cell types. Similarly, the proportion of infected macrophages that are releasing infection virions is much smaller than the proportion of a comparably infected mouse embryo cell culture. Tissue culture passage of MCMV first enhanced (after one passage) and then reduced the infectivity of the virus for macrophages in vitro. The delayed time course and lesser production at early intervals after infection of macrophage cultures could not be attributed to demonstrable inhibitors or to replication in contaminating fibroblasts in the macrophage cultures.
由于关于小鼠巨噬细胞对小鼠巨细胞病毒（MCMV）感染和复制的易感性存在不同结论，我们对巨噬细胞的MCMV感染与允许性细胞系小鼠胚胎细胞的MCMV感染进行了详细比较。尽管这两种细胞系都经历MCMV的增殖性感染，但病毒复制的某些方面存在显著差异，这可能解释了关于巨噬细胞中MCMV周期的一些不同结论。虽然两种细胞系在感染后都产生MCMV，但感染的时间进程在不同细胞类型之间有显著差异。同样，释放感染性病毒粒子的受感染巨噬细胞比例远小于同等感染的小鼠胚胎细胞培养物的比例。MCMV的组织培养传代首先增强了（传代一次后）然后降低了病毒对体外巨噬细胞的感染性。巨噬细胞培养物在感染后早期的延迟时间进程和较低产量不能归因于可证明的抑制剂或巨噬细胞培养物中污染的成纤维细胞中的复制。
