鼠伤寒沙门氏菌中氨同化酶的调控
Regulation of the ammonia assimilatory enzymes in Salmonella typhimurium.
作者信息
Brenchley J E, Baker C A, Patil L G
出版信息
J Bacteriol. 1975 Oct;124(1):182-9. doi: 10.1128/jb.124.1.182-189.1975.
Abstract
The regulation of glutamate dehydrogenase (EC 1.4.1.4), glutamine synthetase (EC 6.3.1.2), and glutamate synthase (EC 2.6.1.53) was examined for cultures of Salmonella typhimurium grown with various nitrogen and amino acid sources. In contrast to the regulatory pattern observed in Klebsiella aerogenes, the glutamate dehydrogenase levels of S. typhimurium do not decrease when glutamine synthetase is derepressed during growth with limiting ammonia. Thus, it appears that the S. typhimurium glutamine synthetase does not regulate the synthesis of glutamate dehydrogenase as reported for K. aerogenes. The glutamate dehydrogenase activity does increase, however, during growth of a glutamate auxotroph with glutamate as a limiting amino acid source. The regulation of glutamate synthase levels is complex with the enzyme activity decreasing during growth with glutamate as a nitrogen source, and during growth of auxotrophs with either glutamine or glutamate as limiting amino acids.
摘要
研究了鼠伤寒沙门氏菌在含有各种氮源和氨基酸源的培养基中生长时，谷氨酸脱氢酶（EC 1.4.1.4）、谷氨酰胺合成酶（EC 6.3.1.2）和谷氨酸合酶（EC 2.6.1.53）的调控情况。与产气克雷伯菌中观察到的调控模式不同，当鼠伤寒沙门氏菌在氨受限的情况下生长且谷氨酰胺合成酶去阻遏时，其谷氨酸脱氢酶水平并不会降低。因此，鼠伤寒沙门氏菌的谷氨酰胺合成酶似乎并不像产气克雷伯菌那样调控谷氨酸脱氢酶的合成。然而，当谷氨酸营养缺陷型菌株以谷氨酸作为限制性氨基酸源生长时，谷氨酸脱氢酶活性确实会增加。谷氨酸合酶水平的调控较为复杂，当以谷氨酸作为氮源生长时，以及营养缺陷型菌株以谷氨酰胺或谷氨酸作为限制性氨基酸生长时，该酶的活性都会降低。
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Protein measurement with the Folin phenol reagent.使用福林酚试剂进行蛋白质测定。
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