缺乏谷氨酸脱氢酶的产气克雷伯菌突变体。
Mutants of Klebsiella aerogenes lacking glutamate dehydrogenase.
作者信息
Brenchley J E, Magasanik B
出版信息
J Bacteriol. 1974 Feb;117(2):544-50. doi: 10.1128/jb.117.2.544-550.1974.
Abstract
A mutant of Klebsiella aerogenes lacking glutamate synthase activity (asm-200) is blocked in only one pathway of glutamate synthesis and can still use glutamate dehydrogenase to produce glutamate when ammonia in sufficient concentration, i.e., higher than 1 mM, is provided in the medium. However, a mutant that has neither glutamate synthase nor glutamate dehydrogenase activities (asm-200, gdhD1) requires glutamate. Transductants obtained by phage grown on wild-type cells of this double mutant, selected on medium containing less than 1 mM ammonia, regain glutamate synthase but not glutamate dehydrogenase. Surprisingly, these gdhD1 transductants grow as well in a variety of media as does a strain with glutamate dehydrogenase activity. Furthermore, transductions with these and other mutants indicate that the genes encoding glutamate synthase, glutamate dehydrogenase, glutamine synthetase, and citrate synthase are not closely linked.
摘要
产气克雷伯菌的一个缺乏谷氨酸合酶活性的突变体（asm - 200）仅在谷氨酸合成的一条途径中受阻，并且当培养基中提供足够浓度（即高于1 mM）的氨时，它仍可利用谷氨酸脱氢酶产生谷氨酸。然而，一个既没有谷氨酸合酶也没有谷氨酸脱氢酶活性的突变体（asm - 200，gdhD1）则需要谷氨酸。通过在这种双突变体的野生型细胞上生长的噬菌体获得的转导子，在含有低于1 mM氨的培养基上进行选择，恢复了谷氨酸合酶活性但未恢复谷氨酸脱氢酶活性。令人惊讶的是，这些gdhD1转导子在各种培养基中的生长情况与具有谷氨酸脱氢酶活性的菌株一样好。此外，用这些及其他突变体进行的转导表明，编码谷氨酸合酶、谷氨酸脱氢酶、谷氨酰胺合成酶和柠檬酸合酶的基因并非紧密连锁。
相似文献
1
Mutants of Klebsiella aerogenes lacking glutamate dehydrogenase.缺乏谷氨酸脱氢酶的产气克雷伯菌突变体。
J Bacteriol. 1974 Feb;117(2):544-50. doi: 10.1128/jb.117.2.544-550.1974.
2
Glutamate dehydrogenase: genetic mapping and isolation of regulatory mutants of Klebsiella aerogenes.谷氨酸脱氢酶：产气克雷伯菌的遗传图谱绘制及调控突变体的分离
J Bacteriol. 1976 Jul;127(1):141-8. doi: 10.1128/jb.127.1.141-148.1976.
3
Effect of methionine sulfoximine and methionine sulfone on glutamate synthesis in Klebsiella aerogenes.蛋氨酸亚砜亚胺和蛋氨酸砜对产气克雷伯菌中谷氨酸合成的影响。
J Bacteriol. 1973 May;114(2):666-73. doi: 10.1128/jb.114.2.666-673.1973.
4
Regulation of nitrogen metabolism in Escherichia coli and Klebsiella aerogenes: studies with the continuous-culture technique.大肠杆菌和产气克雷伯菌中氮代谢的调控：连续培养技术研究
J Bacteriol. 1975 Aug;123(2):407-18. doi: 10.1128/jb.123.2.407-418.1975.
5
Glutamine synthetase as a regulator of enzyme synthesis.谷氨酰胺合成酶作为酶合成的调节因子。
Curr Top Cell Regul. 1974;8(0):119-38. doi: 10.1016/b978-0-12-152808-9.50010-9.
6
Regulation of the ammonia assimilatory enzymes in Salmonella typhimurium.鼠伤寒沙门氏菌中氨同化酶的调控
J Bacteriol. 1975 Oct;124(1):182-9. doi: 10.1128/jb.124.1.182-189.1975.
7
Regulation of the synthesis of enzymes responsible for glutamate formation in Klebsiella aerogenes.产气克雷伯菌中负责谷氨酸形成的酶合成的调控。
J Biol Chem. 1973 Sep 10;248(17):6122-8.
8
Identification of the structural gene for glutamine synthetase in Klebsiella aerogenes.产气克雷伯氏菌中谷氨酰胺合成酶结构基因的鉴定。
J Bacteriol. 1975 Jan;121(1):313-9. doi: 10.1128/jb.121.1.313-319.1975.
9
Ammonia-sensitive mutant of Klebsiella aerogenes.产气克雷伯菌的氨敏感突变体。
J Bacteriol. 1976 May;126(2):739-42. doi: 10.1128/jb.126.2.739-742.1976.
10
Growth of Pseudomonas aeruginosa mutants lacking glutamate synthase activity.缺乏谷氨酸合酶活性的铜绿假单胞菌突变体的生长
J Bacteriol. 1981 Jul;147(1):193-7. doi: 10.1128/jb.147.1.193-197.1981.
引用本文的文献
1
Synchrony Degree of Dietary Energy and Nitrogen Release Influences Microbial Community, Fermentation, and Protein Synthesis in a Rumen Simulation System.日粮能量与氮释放同步程度对瘤胃模拟系统中微生物群落、发酵及蛋白质合成的影响
Microorganisms. 2020 Feb 9;8(2):231. doi: 10.3390/microorganisms8020231.
2
Nitrogen assimilation in Escherichia coli: putting molecular data into a systems perspective.大肠杆菌中的氮同化：将分子数据置于系统视角下。
Microbiol Mol Biol Rev. 2013 Dec;77(4):628-95. doi: 10.1128/MMBR.00025-13.
3
Growth inhibition caused by overexpression of the structural gene for glutamate dehydrogenase (gdhA) from Klebsiella aerogenes.产气克雷伯菌谷氨酸脱氢酶结构基因（gdhA）过表达引起的生长抑制。
J Bacteriol. 2001 Apr;183(8):2709-14. doi: 10.1128/JB.183.8.2709-2714.2001.
4
Helicobacter pylori physiology predicted from genomic comparison of two strains.通过两株幽门螺杆菌菌株的基因组比较预测其生理学特性。
Microbiol Mol Biol Rev. 1999 Sep;63(3):675-707. doi: 10.1128/MMBR.63.3.675-707.1999.
5
The presence of glutamate dehydrogenase is a selective advantage for the Cyanobacterium synechocystis sp. strain PCC 6803 under nonexponential growth conditions.谷氨酸脱氢酶的存在对于聚球藻属蓝细菌PCC 6803菌株在非指数生长条件下是一种选择性优势。
J Bacteriol. 1999 Feb;181(3):808-13. doi: 10.1128/JB.181.3.808-813.1999.
6
Alanine catabolism in Klebsiella aerogenes: molecular characterization of the dadAB operon and its regulation by the nitrogen assimilation control protein.产气克雷伯菌中的丙氨酸分解代谢：dadAB操纵子的分子特征及其受氮同化控制蛋白的调控
J Bacteriol. 1998 Feb;180(3):563-70. doi: 10.1128/JB.180.3.563-570.1998.
7
Glutamine synthetase/glutamate synthase ammonium-assimilating pathway in Schizosaccharomyces pombe.粟酒裂殖酵母中的谷氨酰胺合成酶/谷氨酸合酶铵同化途径。
Curr Microbiol. 1995 Jun;30(6):367-72. doi: 10.1007/BF00369864.
8
Temperature-sensitive glutamate dehydrogenase mutants of Salmonella typhimurium.鼠伤寒沙门氏菌的温度敏感型谷氨酸脱氢酶突变体。
J Bacteriol. 1980 Dec;144(3):1043-7. doi: 10.1128/jb.144.3.1043-1047.1980.
9
Salmonella typhimurium mutants with altered glutamate dehydrogenase and glutamate synthase activities.具有改变的谷氨酸脱氢酶和谷氨酸合酶活性的鼠伤寒沙门氏菌突变体。
J Bacteriol. 1980 Jan;141(1):190-8. doi: 10.1128/jb.141.1.190-198.1980.
10
Cloning and characterization of gdhA, the structural gene for glutamate dehydrogenase of Salmonella typhimurium.鼠伤寒沙门氏菌谷氨酸脱氢酶结构基因gdhA的克隆与特性分析
J Bacteriol. 1984 Jan;157(1):171-8. doi: 10.1128/jb.157.1.171-178.1984.
本文引用的文献
1
Protein measurement with the Folin phenol reagent.使用福林酚试剂进行蛋白质测定。
J Biol Chem. 1951 Nov;193(1):265-75.
2
Conversion of ammonia to amino groups in Escherichia coli.大肠杆菌中氨向氨基的转化。
J Bacteriol. 1960 Sep;80(3):285-8. doi: 10.1128/jb.80.3.285-288.1960.
3
Induction and repression of the histidine-degrading enzymes in Aerobacter aerogenes.产气气杆菌中组氨酸降解酶的诱导与阻遏
J Biol Chem. 1965 Nov;240(11):4320-4.
4
Transduction in Klebsiella.肺炎克雷伯菌中的转导
Nature. 1969 Feb 1;221(5179):475-6. doi: 10.1038/221475a0.
5
'Glutamine(amide):2-oxoglutarate amino transferase oxido-reductase (NADP); an enzyme involved in the synthesis of glutamate by some bacteria.谷氨酰胺（酰胺）：2-氧化戊二酸氨基转移酶氧化还原酶（NADP）；一种参与某些细菌合成谷氨酸的酶。
J Gen Microbiol. 1970 Dec;64(2):187-94. doi: 10.1099/00221287-64-2-187.
6
Genetic and metabolic control of enzymes responsible for histidine degradation in Salmonella typhimurium. 4-imidazolone-5-propionate amidohydrolase and N-formimino-L-glutamate formiminohydrolase.鼠伤寒沙门氏菌中负责组氨酸降解的酶的遗传和代谢调控。4-咪唑酮-5-丙酸酰胺水解酶和N-甲酰亚氨基-L-谷氨酸甲酰亚氨基水解酶。
J Biol Chem. 1971 May 25;246(10):3320-9.
7
The regulation of glutamine synthesis in microorganisms.微生物中谷氨酰胺合成的调控。
Annu Rev Microbiol. 1970;24:501-24. doi: 10.1146/annurev.mi.24.100170.002441.
8
A glutamate-dependent phenotype in E. coli K12: the result of two mutations.大肠杆菌K12中一种依赖谷氨酸的表型：两个突变的结果。
Biochem Biophys Res Commun. 1972 Jun 28;47(6):1498-503. doi: 10.1016/0006-291x(72)90242-2.
9
Resistance to catabolite repression of histidase and proline oxidase during nitrogen-limited growth of Klebsiella aerogenes.产气克雷伯氏菌在氮限制生长期间对组氨酸酶和脯氨酸氧化酶分解代谢阻遏的抗性。
J Biol Chem. 1971 Oct 25;246(20):6288-96.
10
Regulation of the synthesis of enzymes responsible for glutamate formation in Klebsiella aerogenes.产气克雷伯菌中负责谷氨酸形成的酶合成的调控。
J Biol Chem. 1973 Sep 10;248(17):6122-8.
Zotero 插件