[氨基酸对多粘芽孢杆菌1538培养物生长及多粘菌素B生物合成的影响]

[Effect of amino acids on the growth of a B. polymyza 1538 culture and the biosynthesis of polymyxin B].

作者信息

Pavliuk Iu V, Bogatskii M A, Orlova N V, Anakhova V A

出版信息

Antibiotiki. 1979 Nov;24(11):815-20.

Abstract

The effect of a number of the most important amino acids on the growth of B. polymyxa 1538 and polymyxin B biosynthesis was studied. It was found that all of the amino acids tested except D-L-threonin and L-alpha-gamma-DABA inhibited the initial growth of the organism. D-L-threonine and L-alpha-gamma-DABA had some stimulating effect on the culture growth without affecting the final accumulation of the biomass. The antibiotic synthesis proceeded more vigorously when the medium contained D-L-threonine, L-alpha-gamma-DABA, L-asparagine, L-proline, L-glutamine, D-L-asparaginic acid and L-glutamic acid in the concentrations tested. Neither of the tested amino acids used alone or in combination provided the levels of polymyxin B biosynthesis observed on the media containing rich sources of organic nitrogen.

摘要

研究了多种最重要的氨基酸对多粘芽孢杆菌1538生长及多粘菌素B生物合成的影响。结果发现，除D-L-苏氨酸和L-α-γ-二氨基丁酸外，所有测试的氨基酸均抑制该菌的初始生长。D-L-苏氨酸和L-α-γ-二氨基丁酸对培养物生长有一定刺激作用，但不影响生物量的最终积累。当培养基中含有测试浓度的D-L-苏氨酸、L-α-γ-二氨基丁酸、L-天冬酰胺、L-脯氨酸、L-谷氨酰胺、D-L-天冬氨酸和L-谷氨酸时，抗生素合成更为旺盛。单独使用或组合使用的任何一种测试氨基酸都无法达到在含有丰富有机氮源的培养基上观察到的多粘菌素B生物合成水平。