α-α'-联吡啶或邻菲罗啉对枯草芽孢杆菌孢子中可溶性还原型烟酰胺腺嘌呤二核苷酸氧化酶的刺激作用以及二吡啶甲酸对受刺激酶的抑制作用。
Alpha-alpha'-dipyridyl or ortho-phenanthroline stimulation of the soluble reduced nicotinamide adenine dinucleotide oxidase from Bacillus subtilis spores and dipicolinic acid inhibition of the stimulated enzymes.
作者信息
Tochikubo K
出版信息
J Bacteriol. 1974 Mar;117(3):1017-22. doi: 10.1128/jb.117.3.1017-1022.1974.
Abstract
Soluble reduced nicotinamide adenine dinucleotide oxidase activity in extracts of Bacillus subtilis spores was stimulated by the addition of not only flavine mononucleotide (FMN) or flavine adenine dinucleotide (FAD) but also alpha-alpha'-dipyridyl or o-phenanthroline. These chelating agents showed stronger effect on the enzyme from spores than on that from vegetative cells. Activity stimulated by alpha-alpha'-dipyridyl or o-phenanthroline was inhibited by atabrine or dipicolinic acid, whereas FMN or FAD stimulation was inhibited only by atabrine.
摘要
枯草芽孢杆菌孢子提取物中的可溶性还原型烟酰胺腺嘌呤二核苷酸氧化酶活性，不仅可通过添加黄素单核苷酸（FMN）或黄素腺嘌呤二核苷酸（FAD）来刺激，还可通过添加α-α'-联吡啶或邻菲罗啉来刺激。这些螯合剂对孢子中的酶的作用比对营养细胞中的酶的作用更强。由α-α'-联吡啶或邻菲罗啉刺激产生的活性可被阿的平或二吡啶甲酸抑制，而FMN或FAD刺激产生的活性仅被阿的平抑制。
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