通过亲和层析法纯化铜绿假单胞菌外毒素。
Purification of Pseudomonas aeruginosa exotoxin by affinity chromatography.
作者信息
Taylor N S, Pollack M
出版信息
Infect Immun. 1978 Jan;19(1):66-70. doi: 10.1128/iai.19.1.66-70.1978.
Abstract
Pseudomonas aeruginosa exotoxin A was purified by affinity chromatography from culture supernatants by elution of toxin from antitoxin immunoglobulin G-Sepharose 4B with 3 M NaSCN. The purity, toxicity, and enzymatic activity of exotoxin obtained were comparable to those of toxin purified by previously reported multiple-step procedures.
摘要
铜绿假单胞菌外毒素A通过亲和层析法从培养上清液中纯化得到，具体操作是用3 M硫氰酸钠从抗毒素免疫球蛋白G-琼脂糖凝胶4B上洗脱毒素。所得外毒素的纯度、毒性和酶活性与先前报道的多步纯化法所获毒素相当。
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