混合功能胺氧化酶在仓鼠肝微粒体制剂对2-乙酰氨基芴进行N-羟基化反应中的作用。
Role of mixed-function amine oxidases in N-hydroxylation of 2-acetamidofluorene by hamster liver microsomal preparations.
作者信息
Lotlikar P D, Wertman K, Luha L
出版信息
Biochem J. 1973 Dec;136(4):1137-40. doi: 10.1042/bj1361137.
Abstract
Pretreatment of hamsters with 3-methylcholanthrene (100mg/kg body wt.) 24h before death did not appreciably change the extent of N-oxide formation when hepatic microsomal preparations were incubated with NN-dimethylaniline as substrate. In contrast, the N-hydroxylation of 2-acetamidofluorene was increased severalfold in hepatic microsomal preparations from pretreated animals. Under these conditions there were no appreciable changes in cytochrome P-450 content and NADPH-cytochrome c reductase activity. On the basis of these comparative data, it is suggested that amine oxidase is not involved in N-hydroxylation of 2-acetamidofluorene.
摘要
在仓鼠死亡前24小时用3-甲基胆蒽（100mg/kg体重）进行预处理，当肝微粒体制剂与NN-二甲基苯胺作为底物一起孵育时，N-氧化物形成的程度没有明显变化。相比之下，来自预处理动物的肝微粒体制剂中2-乙酰氨基芴的N-羟基化增加了几倍。在这些条件下，细胞色素P-450含量和NADPH-细胞色素c还原酶活性没有明显变化。基于这些比较数据，表明胺氧化酶不参与2-乙酰氨基芴的N-羟基化。
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