一氧化碳对仓鼠肝微粒体制剂中2-乙酰氨基芴的N-羟基化和环羟基化的抑制作用。
Inhibitory effect of carbon monoxide on the N- and ring-hydroxylation of 2-acetamidofluorene by hamster hepatic microsomal preparations.
作者信息
Lotlikar P D, Zaleski K
出版信息
Biochem J. 1974 Nov;144(2):427-30. doi: 10.1042/bj1440427.
Abstract
The effect of CO on N-, 3-, 5- and 7-hydroxylation of 2-acetamidofluorene by liver microsomal fractions from control and 3-methylcholanthrene-pretreated hamsters was studied. All hydroxylations were inhibited by CO, but the degree of inhibition was different for each hydroxylation. The ratios of CO to O(2) needed for 50% inhibition of the N-, 3-, 5- and 7-hydroxylations by control preparations were 8.0:1, 8.2:1, 4.2:1 and 7.1:1 respectively and by preparations from treated animals were 4.2:1, 8.9:1, 2.3:1 and 3.2:1 respectively. These results are discussed in terms of the possible presence of more than one type of cytochrome P-450 involved in hydroxylations of 2-acetamidofluorene by liver microsomal fractions from both control and pretreated hamsters.
摘要
研究了一氧化碳（CO）对来自对照仓鼠和经3-甲基胆蒽预处理的仓鼠肝脏微粒体组分中2-乙酰氨基芴的N-、3-、5-和7-羟基化作用的影响。所有羟基化作用均受到CO的抑制，但每种羟基化作用的抑制程度不同。对照制剂对N-、3-、5-和7-羟基化作用50%抑制所需的CO与O₂的比例分别为8.0:1、8.2:1、4.2:1和7.1:1，而经处理动物的制剂该比例分别为4.2:1、8.9:1、2.3:1和3.2:1。根据可能存在不止一种细胞色素P-450参与对照仓鼠和预处理仓鼠肝脏微粒体组分对2-乙酰氨基芴的羟基化作用这一情况，对这些结果进行了讨论。
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