干酪乳杆菌ATCC 7469简化叶酸检测法优于氯霉素适应菌株检测法。
Superiority of simplified assay for folate with Lactobacillus casei ATCC 7469 over assay with chloramphenicol-adapted strain.
作者信息
Cooper B A
出版信息
J Clin Pathol. 1973 Dec;26(12):963-7. doi: 10.1136/jcp.26.12.963.
Abstract
Direct assay of serum folate by mixing serum with a mixture of L. casei and medium without autoclaving was found to provide serum folate results similar to those by extraction of serum with heat. This procedure was found more reliable than that using the same technique with chloramphenicol and a strain of L. casei adapted to grow in this material. A portion of apparent folate activity assayed in haemolysates by such direct assay was found to be due to material other than folate. It is recommended that heat extraction of haemolysates be continued for determination of whole blood folate, or that an additional standardization of technique be included if direct assay is used.
摘要
通过将血清与干酪乳杆菌和未高压灭菌的培养基混合来直接测定血清叶酸，发现其提供的血清叶酸结果与用加热法提取血清的结果相似。发现该方法比使用相同技术并添加氯霉素以及使用适应在此种材料中生长的干酪乳杆菌菌株的方法更可靠。通过这种直接测定法在溶血产物中测定的一部分表观叶酸活性被发现是由叶酸以外的物质引起的。建议继续采用加热提取溶血产物的方法来测定全血叶酸，或者如果使用直接测定法，则应包括额外的技术标准化。
相似文献
1
Superiority of simplified assay for folate with Lactobacillus casei ATCC 7469 over assay with chloramphenicol-adapted strain.干酪乳杆菌ATCC 7469简化叶酸检测法优于氯霉素适应菌株检测法。
J Clin Pathol. 1973 Dec;26(12):963-7. doi: 10.1136/jcp.26.12.963.
2
Experience with microbiological assay for folate using a chloramphenicol-resistant L. casei strain.使用氯霉素抗性干酪乳杆菌菌株进行叶酸微生物测定的经验。
J Clin Pathol. 1972 Dec;25(12):1050-2. doi: 10.1136/jcp.25.12.1050.
3
Continuous-flow automation of the Lactobacillus casei serum folate assay.干酪乳杆菌血清叶酸测定的连续流动自动化
J Clin Pathol. 1977 Dec;30(12):1168-74. doi: 10.1136/jcp.30.12.1168.
4
Studies on the response of Lactobacillus casei to different folate monoglutamates.干酪乳杆菌对不同叶酸单谷氨酸盐反应的研究。
Br J Nutr. 1982 Mar;47(2):183-9. doi: 10.1079/bjn19820026.
5
A comparison of seven strains of Lactobacillus casei var. rhamnosus in relation to their use in the microbiological assay of serum folate.
J Appl Bacteriol. 1974 Sep;37(3):373-89. doi: 10.1111/j.1365-2672.1974.tb00453.x.
6
Cryoprotected Lactobacillus casei: an approach to standardization of microbiological assay of folic acid in serum.
Clin Chem. 1981 May;27(5):745-7.
7
Automation of the assay of folate in serum and whole blood.血清和全血中叶酸测定的自动化
J Clin Pathol. 1970 Feb;23(1):54-9. doi: 10.1136/jcp.23.1.54.
8
The effect of different serum concentrations of antimicrobial agents on the Lactobacillus casei folate assay.不同血清浓度的抗菌剂对干酪乳杆菌叶酸测定的影响。
Acta Pathol Microbiol Scand B Microbiol Immunol. 1974 Jun;82(3):371-4.
9
Effect of Tween 80 on incubation period of Lactobacillus casei assay of serum folate.吐温80对血清叶酸酪酸梭状芽孢杆菌检测潜伏期的影响。
J Clin Pathol. 1978 Mar;31(3):227-9. doi: 10.1136/jcp.31.3.227.
10
New Tween-80 microbiological assay of serum folate levels in humans and animals.人及动物血清叶酸水平的新型吐温 - 80微生物学检测法
J AOAC Int. 2012 Sep-Oct;95(5):1505-10. doi: 10.5740/jaoacint.11-071.
引用本文的文献
1
Late-life dietary folate restriction reduces biosynthesis without compromising healthspan in mice.晚年饮食叶酸限制减少生物合成而不损害小鼠健康寿命。
Life Sci Alliance. 2024 Jul 23;7(10). doi: 10.26508/lsa.202402868. Print 2024 Oct.
2
Late-life dietary folate restriction reduces biosynthetic processes without compromising healthspan in mice.晚年饮食中叶酸限制可减少生物合成过程，且不影响小鼠的健康寿命。
bioRxiv. 2024 Jan 12:2024.01.12.575290. doi: 10.1101/2024.01.12.575290.
3
Detrimental health relationship between blood lead and cadmium and the red blood cell folate level.血铅、血镉与红细胞叶酸水平之间对健康有害的关系。
Sci Rep. 2022 Apr 22;12(1):6628. doi: 10.1038/s41598-022-10562-9.
4
An explanation of the decreased serum folate values found using methods that involve prior extraction of the serum.解释了使用需要预先提取血清的方法时发现的血清叶酸值降低的原因。
Ir J Med Sci. 1975 Dec;144(1):395-8. doi: 10.1007/BF02939044.
5
Nutrition following gastric operations for morbid obesity.病态肥胖症胃手术后的营养
Ann Surg. 1983 Sep;198(3):347-55. doi: 10.1097/00000658-198309000-00011.
6
Intracellular folate distribution in cultured fibroblasts from patients with the fragile X syndrome.脆性X综合征患者培养成纤维细胞内叶酸的分布情况。
Am J Hum Genet. 1983 Sep;35(5):869-78.
7
A critical intracellular concentration of fully reduced non-methylated folate polyglutamates prevents macrocytosis and diminished growth rate of human cell line K562 in culture.完全还原的非甲基化叶酸多聚谷氨酸的关键细胞内浓度可防止人细胞系K562在培养中出现大细胞性贫血并减缓其生长速率。
Biochem J. 1983 Aug 15;214(2):465-70. doi: 10.1042/bj2140465.
8
Folic acid blinded trial in identical twins with fragile X syndrome.脆性X综合征同卵双胞胎的叶酸盲法试验。
Am J Hum Genet. 1985 May;37(3):543-52.
9
Nutrition after vertical banded gastroplasty.垂直束带胃成形术后的营养
Ann Surg. 1987 Nov;206(5):555-63. doi: 10.1097/00000658-198711000-00001.
10
A regional quality control trial of folate assays.一项叶酸检测的区域质量控制试验。
J Clin Pathol. 1977 Apr;30(4):372-4. doi: 10.1136/jcp.30.4.372.
本文引用的文献
1
A microbiologic method for detecting folic acid deficiency in man.一种检测人体叶酸缺乏的微生物学方法。
Clin Chem. 1959 Aug;5(4):275-80.
2
Aseptic addition method for Lactobacillus casei assay of folate activity in human serum.用于检测人血清中叶酸活性的干酪乳杆菌测定的无菌添加法。
J Clin Pathol. 1966 Jan;19(1):12-6. doi: 10.1136/jcp.19.1.12.
3
Effect of incubation time on the L. casei bioassay of folic acid in serum.
Blood. 1969 Aug;34(2):216-21.
4
Automation of the assay of folate in serum and whole blood.血清和全血中叶酸测定的自动化
J Clin Pathol. 1970 Feb;23(1):54-9. doi: 10.1136/jcp.23.1.54.
5
An automated method for the measurement of folate activity.一种用于测量叶酸活性的自动化方法。
J Clin Pathol. 1970 Feb;23(1):47-53. doi: 10.1136/jcp.23.1.47.
6
Radioisotopic assay for measurement of serum folate levels.用于测量血清叶酸水平的放射性同位素测定法。
Blood. 1971 Aug;38(2):219-28.
7
Studies of (3H)folic acid uptake by Lactobacillus casei.干酪乳杆菌对（3H）叶酸摄取的研究。
Biochim Biophys Acta. 1970 Apr 14;208(1):99-109. doi: 10.1016/0304-4165(70)90052-8.
8
A comparison of serum folate estimations using two different methods.使用两种不同方法对血清叶酸估计值进行的比较。
J Clin Pathol. 1973 Jan;26(1):80-1. doi: 10.1136/jcp.26.1.80.
9
Experience with microbiological assay for folate using a chloramphenicol-resistant L. casei strain.使用氯霉素抗性干酪乳杆菌菌株进行叶酸微生物测定的经验。
J Clin Pathol. 1972 Dec;25(12):1050-2. doi: 10.1136/jcp.25.12.1050.
Zotero 插件