Hemolytic anti-hr" (e) detectable solely by an automated polybrene technique.

An example of a hemolytic anti-hr" (e) detectable solely by an AutoAnalyzer-Polybrene (AAP) system is reported. The antibody was undetectable by routine indirect antiglobulin and enzyme tests, and produced a delayed intravascular hemolytic transfusion reaction. The anti-hr" (e) was no longer AAP detectable five months after the transfusion reaction. This paper points out the potential usefulness of the AAP technique in detecting alloantibodies in cases of transfusion reactions where no antibody specificity can be demonstrated by standard manual techniques.