内源性还原型烟酰胺腺嘌呤二核苷酸对大肠杆菌膜囊泡转运的刺激作用。
Stimulation of transport into Escherichia coli membrane vesicles by internally generated reduced nictotinamide adenine dinucleotide.
作者信息
Futai M
出版信息
J Bacteriol. 1974 Nov;120(2):861-5. doi: 10.1128/jb.120.2.861-865.1974.
Abstract
Membrane vesicles containing internal alcohol dehydrogenase and nicotinamide adenine dinucleotide (NAD) were prepared from Escherichia coli ML308-225. Upon the addition of ethanol, these vesicles respired and transported several amino acids. Transport rates driven by internally generated reduced NAD (NADH) were about 60 to 80% of that stimulated by d-lactate. This transport was inhibited by cyanide and anaerobiosis. Ferricyanide, a nonpermeable electron acceptor, inhibited transport stimulated by external NADH, but not that stimulated by internally generated NADH.
摘要
从大肠杆菌ML308 - 225制备了含有内部乙醇脱氢酶和烟酰胺腺嘌呤二核苷酸（NAD）的膜囊泡。加入乙醇后，这些膜囊泡进行呼吸并转运几种氨基酸。由内部产生的还原型NAD（NADH）驱动的转运速率约为d - 乳酸刺激的转运速率的60%至80%。这种转运受到氰化物和厌氧环境的抑制。铁氰化物是一种不可渗透的电子受体，它抑制由外部NADH刺激的转运，但不抑制由内部产生的NADH刺激的转运。
相似文献
1
Stimulation of transport into Escherichia coli membrane vesicles by internally generated reduced nictotinamide adenine dinucleotide.内源性还原型烟酰胺腺嘌呤二核苷酸对大肠杆菌膜囊泡转运的刺激作用。
J Bacteriol. 1974 Nov;120(2):861-5. doi: 10.1128/jb.120.2.861-865.1974.
2
Functional mosaicism of membrane proteins in vesicles of Escherichia coli.大肠杆菌囊泡中膜蛋白的功能镶嵌现象
J Bacteriol. 1977 Feb;129(2):959-66. doi: 10.1128/jb.129.2.959-966.1977.
3
Ubiquinone-mediated coupling of NADH dehydrogenase to active transport in membrane vesicles from Escherichia coli.泛醌介导的大肠杆菌膜囊泡中NADH脱氢酶与主动运输的偶联。
Proc Natl Acad Sci U S A. 1975 Oct;72(10):3970-4. doi: 10.1073/pnas.72.10.3970.
4
5
Amino acid transport in membrane vesicles of obligately anaerobic Veillonella alcalescens.专性厌氧产碱韦荣球菌膜囊泡中的氨基酸转运
J Bacteriol. 1975 Apr;122(1):245-9. doi: 10.1128/jb.122.1.245-249.1975.
6
Relation between reduced nicotinamide adenine dinucleotide oxidation and amino acid transport in membrane vesicles from Bacillus subtilis.枯草芽孢杆菌膜泡中还原型烟酰胺腺嘌呤二核苷酸氧化与氨基酸转运的关系
J Bacteriol. 1975 Mar;121(3):807-13. doi: 10.1128/jb.121.3.807-813.1975.
7
Lipophilic chelator inhibition of electron transport in Escherichia coli.亲脂性螯合剂对大肠杆菌电子传递的抑制作用。
J Bacteriol. 1975 May;122(2):686-90. doi: 10.1128/jb.122.2.686-690.1975.
8
9
Reconstitution of reduced nicotinamide adenine dinucleotide oxidase activity with menadione in membrane vesicles from the menaquinone-deficient Bacillus subtilis aro D. Relation between electron transfer and active transport.用甲萘醌在缺乏甲基萘醌的枯草芽孢杆菌aro D的膜泡中重建还原型烟酰胺腺嘌呤二核苷酸氧化酶活性。电子传递与主动运输之间的关系。
Eur J Biochem. 1976 Aug 16;67(2):357-65. doi: 10.1111/j.1432-1033.1976.tb10699.x.
10
Nature of the specificity of alcohol coupling to L-alanine transport into isolated membrane vesicles of a marine pseudomonad.酒精与L-丙氨酸转运至海洋假单胞菌分离膜囊泡过程中特异性的本质。
J Bacteriol. 1974 Mar;117(3):1043-54. doi: 10.1128/jb.117.3.1043-1054.1974.
引用本文的文献
1
Our research on proton pumping ATPases over three decades: their biochemistry, molecular biology and cell biology.我们对质子泵 ATP 酶的研究已有三十余年：它们的生物化学、分子生物学和细胞生物学。
Proc Jpn Acad Ser B Phys Biol Sci. 2007 Jan;82(10):416-38. doi: 10.2183/pjab.82.416. Epub 2007 Jan 12.
2
Biochemical and structural studies of NADH-dependent FabG used to increase the bacterial production of fatty acids under anaerobic conditions.对依赖NADH的FabG进行生化和结构研究，以提高厌氧条件下细菌脂肪酸的产量。
Appl Environ Microbiol. 2014 Jan;80(2):497-505. doi: 10.1128/AEM.03194-13. Epub 2013 Nov 8.
3
Energy transduction by electron transfer via a pyrrolo-quinoline quinone-dependent glucose dehydrogenase in Escherichia coli, Pseudomonas aeruginosa, and Acinetobacter calcoaceticus (var. lwoffi).大肠杆菌、铜绿假单胞菌和乙酸钙不动杆菌（鲁氏变种）中通过依赖于吡咯并喹啉醌的葡萄糖脱氢酶进行电子转移的能量转换
J Bacteriol. 1985 Aug;163(2):493-9. doi: 10.1128/jb.163.2.493-499.1985.
4
The reversibility of active sulphate transport in membrane vesicles of Paracoccus denitrificans.反硝化副球菌膜囊泡中活性硫酸盐转运的可逆性
Biochem J. 1975 Sep;150(3):527-36. doi: 10.1042/bj1500527.
5
Localization of D-lactate dehydrogenase in membrane vesicles prepared by using a french press or ethylenediaminetetraacetate-lysozyme from Escherichia coli.利用法国压榨机或来自大肠杆菌的乙二胺四乙酸-溶菌酶制备的膜囊泡中D-乳酸脱氢酶的定位
J Bacteriol. 1975 Oct;124(1):470-5. doi: 10.1128/jb.124.1.470-475.1975.
6
Internally generated reduced nicotinamide adenine dinucleotide as a substrate for glycine transport by membrane vesicles of Paracoccus denitrificans.内源生成的还原型烟酰胺腺嘌呤二核苷酸作为反硝化副球菌膜囊泡转运甘氨酸的底物。
J Bacteriol. 1977 Jan;129(1):559-61. doi: 10.1128/jb.129.1.559-561.1977.
7
Adenosine 5'-triphosphate synthesis energized by an artificially imposed membrane potential in membrane vesicles of Escherichia coli.由人为施加的膜电位驱动的大肠杆菌膜囊泡中三磷酸腺苷的合成
J Bacteriol. 1976 Jul;127(1):154-61. doi: 10.1128/jb.127.1.154-161.1976.
本文引用的文献
1
ZINC, A COMPONENT OF YEAST ALCOHOL DEHYDROGENASE.锌，酵母乙醇脱氢酶的一种成分。
Proc Natl Acad Sci U S A. 1955 Jun 15;41(6):327-38. doi: 10.1073/pnas.41.6.327.
2
Protein measurement with the Folin phenol reagent.使用福林酚试剂进行蛋白质测定。
J Biol Chem. 1951 Nov;193(1):265-75.
3
Production and ultrastructure of lysozyme and ethylenediaminetetraacetate-lysozyme spheroplasts of Escherichia coli.大肠杆菌溶菌酶及乙二胺四乙酸 - 溶菌酶原生质球的产生与超微结构
J Bacteriol. 1967 Jan;93(1):427-37. doi: 10.1128/jb.93.1.427-437.1967.
4
Anaerobic transport in Escherichia coli membrane vesicles.大肠杆菌膜囊泡中的厌氧转运
Proc Natl Acad Sci U S A. 1973 Dec;70(12):3376-81. doi: 10.1073/pnas.70.12.3376.
5
Transport across isolated bacterial cytoplasmic membranes.跨分离的细菌细胞质膜转运。
Biochim Biophys Acta. 1972 Aug 4;265(3):367-416. doi: 10.1016/0304-4157(72)90014-7.
6
Explanation for the apparent inefficiency of reduced nicotinamide adenine dinucleotide in energizing amino acid transport in membrane vesicles.关于还原型烟酰胺腺嘌呤二核苷酸在为膜囊泡中的氨基酸转运提供能量方面明显低效的解释。
J Bacteriol. 1974 May;118(2):497-504. doi: 10.1128/jb.118.2.497-504.1974.
7
Evaluation of the chemiosmotic interpretation of active transport in bacterial membrane vesicles.细菌膜囊泡中主动运输的化学渗透解释评估。
Ann N Y Acad Sci. 1974 Feb 18;227:312-27. doi: 10.1111/j.1749-6632.1974.tb14396.x.
8
Transport of lactate and succinate by membrane vesicles of Escherichia coli, Bacillus subtilis and a pseudomonas species.大肠杆菌、枯草芽孢杆菌和一种假单胞菌属细菌的膜囊泡对乳酸盐和琥珀酸盐的转运
Eur J Biochem. 1973 Apr 2;34(1):58-67. doi: 10.1111/j.1432-1033.1973.tb02728.x.
9
10
Mutants of Salmonella typhimurium and Escherichia coli pleiotropically defective in active transport.鼠伤寒沙门氏菌和大肠杆菌在主动运输方面多效性缺陷的突变体。
Proc Natl Acad Sci U S A. 1972 Nov;69(11):3336-40. doi: 10.1073/pnas.69.11.3336.
Zotero 插件