Kosaka H, Imaizumi K, Imai K, Tyuma I
Biochim Biophys Acta. 1979 Nov 23;581(1):184-8. doi: 10.1016/0005-2795(79)90235-6.
During the reaction of oxyhemoglobin (HbO2) with nitrite, the concentration of residual nitrite, nitrate, oxygen, and methemoglobin (Hb+) was determined successively. The results obtained at various pH values indicate the following stoichiometry for the overall reaction: 4HbO2 + 4NO2- 4H+ leads to 4Hb+ + 4NO3- + O2 + 2H2 O (Hb denotes hemoglobin monomer). NO2- binds with methemoglobin noncooperatively with a binding constant of 340 M-1 at pH 7.4 and 25 degrees C. Thus, the major part of Hb+ produced is aquomethemoglobin, not methemoglobin nitrite, when less than 2 equivalents of nitrite is used for the oxidation.
在氧合血红蛋白（HbO₂）与亚硝酸盐的反应过程中，依次测定了残余亚硝酸盐、硝酸盐、氧气和高铁血红蛋白（Hb⁺）的浓度。在不同pH值下获得的结果表明了整个反应的以下化学计量关系：4HbO₂ + 4NO₂⁻ + 4H⁺ → 4Hb⁺ + 4NO₃⁻ + O₂ + 2H₂O（Hb表示血红蛋白单体）。在pH 7.4和25℃时，NO₂⁻与高铁血红蛋白非协同结合，结合常数为340 M⁻¹。因此，当用于氧化的亚硝酸盐少于2当量时，产生的Hb⁺的主要部分是水合高铁血红蛋白，而不是亚硝酸高铁血红蛋白。
