用放线菌素处理的小鼠成纤维细胞中核糖核酸的降解
Degradation of ribonucleic acid in mouse fibroblasts treated with actinomycin.
作者信息
Wiesner R, Acs G, Reich E, Shafiq A
出版信息
J Cell Biol. 1965 Oct;27(1):47-52. doi: 10.1083/jcb.27.1.47.
Abstract
The cellular RNA content of mouse fibroblasts incubated with actinomycin decreases at a rate of about 1 to 1.5 per cent per hour, while DNA and protein content remain unchanged. This degradation affects nuclear and cytoplasmic RNA, ribosomal and soluble RNA. The breakdown products appear quantitatively in the acid-soluble fraction of the cells and the medium. Polynucleotides synthesized a short period (120 minutes) prior to exposure to actinomycin are degraded before those synthesized 8 to 12 hours previously.
摘要
用放线菌素处理的小鼠成纤维细胞的细胞RNA含量以每小时约1%至1.5%的速率下降，而DNA和蛋白质含量保持不变。这种降解影响核RNA和细胞质RNA、核糖体RNA和可溶性RNA。降解产物定量地出现在细胞和培养基的酸溶性部分中。在接触放线菌素之前短时间（120分钟）合成的多核苷酸比8至12小时前合成的多核苷酸先被降解。
相似文献
1
Degradation of ribonucleic acid in mouse fibroblasts treated with actinomycin.用放线菌素处理的小鼠成纤维细胞中核糖核酸的降解
J Cell Biol. 1965 Oct;27(1):47-52. doi: 10.1083/jcb.27.1.47.
2
Degradation of cellular ribonucleic acid in Newcastle disease virus infected cells.新城疫病毒感染细胞中细胞核糖核酸的降解
J Gen Virol. 1969 Mar;4(2):245-51. doi: 10.1099/0022-1317-4-2-245.
3
HYBRIDIZATION OF RAPIDLY LABELED NUCLEAR RIBONUCLEIC ACIDS.快速标记核糖核酸的杂交
Science. 1964 Jul 31;145(3631):504-7. doi: 10.1126/science.145.3631.504.
4
Ribonucleic acid production and breakdown in synchronized erythroid cohorts from actinomycin-treated animals.来自放线菌素处理动物的同步红细胞群体中的核糖核酸产生与分解
J Lab Clin Med. 1971 Jun;77(6):923-30.
5
[Micro-interference and cytophotometry study of the effects of actinomycin D on the synthesis of total nuclear proteins and desoxyribonucleic acids in rat fibroblasts cultivated in vitro].
C R Seances Soc Biol Fil. 1969 Apr 3;162(10):1857-60.
6
Actinomycin D: inhibition of phospholipid synthesis in chick embryo cells.放线菌素D：对鸡胚细胞中磷脂合成的抑制作用。
Science. 1968 Apr 19;160(3825):316-7. doi: 10.1126/science.160.3825.316.
7
[Comparative study of the action of 2 antibiotics, actinomycin and toyocamycin on ribosomal protein synthesis in L cells].[两种抗生素放线菌素和丰加霉素对L细胞核糖体蛋白质合成作用的比较研究]
C R Acad Hebd Seances Acad Sci D. 1970 Jan 19;270(3):543-6.
8
Synthesis of ribonucleic acid in L cells during inhibition of protein synthesis by cycloheximide.在环己酰亚胺抑制蛋白质合成期间L细胞中核糖核酸的合成
Mol Pharmacol. 1966 Nov;2(6):543-57.
9
Studies on the origin of ribosomes in Amoeba proteus.变形虫核糖体起源的研究。
J Cell Biol. 1969 Mar;40(3):622-32. doi: 10.1083/jcb.40.3.622.
10
ROLE OF THE NUCLEOLUS IN RIBONUCLEIC ACID METABOLISM AND OTHER CELLULAR PROCESSES.核仁在核糖核酸代谢及其他细胞过程中的作用。
Natl Cancer Inst Monogr. 1964 May;14:73-89.
引用本文的文献
1
Actinomycin D and the hormonal induction of amylase synthesis in barley aleurone layers.放线菌素 D 和激素诱导大麦糊粉层中淀粉酶的合成。
Planta. 1972 Mar;106(1):1-12. doi: 10.1007/BF00385468.
2
Histological studies on the effect of actinomycin D on retrograde nerve cell reaction in the facial nucleus of mice.放线菌素D对小鼠面神经核逆行性神经细胞反应影响的组织学研究。
Acta Neuropathol. 1967 Oct 20;9(2):146-57. doi: 10.1007/BF00691440.
3
The effect of proflavine on HeLa cells.硫酸原黄素对海拉细胞的作用。
Biochem J. 1966 Aug;100(2):467-72. doi: 10.1042/bj1000467.
4
The loss of rapidly labelled ribonucleic acid from isolated HeLa cell nuclei.从分离的海拉细胞核中快速标记的核糖核酸的损失。
Biochem J. 1969 Mar;112(1):71-9. doi: 10.1042/bj1120071.
5
Kinetics of RNA synthesis in toad bladder epithelium: action of aldosterone during the latent period.蟾蜍膀胱上皮细胞中RNA合成的动力学：潜伏期醛固酮的作用
J Clin Invest. 1971 Mar;50(3):543-51. doi: 10.1172/JCI106523.
6
Stimulation of incorporation of nucleic acid precursors into HeLa cells caused by proflavine.硫酸原黄素引起核酸前体掺入海拉细胞的刺激作用。
Biochem J. 1969 Dec;115(4):823-9. doi: 10.1042/bj1150823.
7
Cytology of cultured cells surviving actinomycin D.经放线菌素D处理后存活的培养细胞的细胞学研究
Proc Natl Acad Sci U S A. 1967 Jun;57(6):1607-10. doi: 10.1073/pnas.57.6.1607.
8
Serum-induced formation of lysosomes in HeLa cells: a process sensitive to actinomycin D.血清诱导HeLa细胞中溶酶体的形成：一个对放线菌素D敏感的过程。
Proc Natl Acad Sci U S A. 1966 Apr;55(4):852-7. doi: 10.1073/pnas.55.4.852.
9
Effect of temperature of aldehyde fixation on the radioautographic localization of ribonucleoprotein in nucleoli of HeLa cells. Inhibition by puromycin and actinomycin D.醛固定温度对HeLa细胞核仁核糖核蛋白放射自显影定位的影响。嘌呤霉素和放线菌素D的抑制作用。
J Cell Biol. 1967 Sep;34(3):721-34. doi: 10.1083/jcb.34.3.721.
10
Actinomycin D-induced phenocopies in Drosophila melanogaster and their relevance to the time of gene action.放线菌素D诱导的黑腹果蝇拟表型及其与基因作用时间的相关性。
Genetica. 1972;43(2):207-22. doi: 10.1007/BF00123627.
本文引用的文献
1
Protein measurement with the Folin phenol reagent.使用福林酚试剂进行蛋白质测定。
J Biol Chem. 1951 Nov;193(1):265-75.
2
Sedimentation characteristics of rapidly labelled RNA from HeLa cells.来自海拉细胞的快速标记RNA的沉降特性。
Biochem Biophys Res Commun. 1962 Jun 4;7:486-90. doi: 10.1016/0006-291x(62)90341-8.
3
Action of actinomycin D on animal cells and viruses.放线菌素D对动物细胞和病毒的作用。
Proc Natl Acad Sci U S A. 1962 Jul 15;48(7):1238-45. doi: 10.1073/pnas.48.7.1238.
4
A fractionating column for analysis of nucleic acids.用于核酸分析的分馏柱。
Anal Biochem. 1960 Jun;1:66-77. doi: 10.1016/0003-2697(60)90020-8.
5
BREAKDOWN OF NUCLEAR RIBONUCLEIC ACID IN THE PRESENCE OF ACTINOMYCIN D.在放线菌素D存在的情况下核糖核酸的分解
Nature. 1964 Jun 27;202:1301-3. doi: 10.1038/2021301a0.
6
EFFECT OF ACTINOMYCIN D ON HELA CELL NUCLEAR RNA METABOLISM.放线菌素D对海拉细胞核RNA代谢的影响。
Proc Soc Exp Biol Med. 1963 Aug-Sep;113:886-9. doi: 10.3181/00379727-113-28521.
7
Rapidly labelled ribonucleic acid in the cell nucleus.细胞核中快速标记的核糖核酸。
Nature. 1963 Apr 13;198:184-5. doi: 10.1038/198184a0.
8
9
Effect of mitomycin C on mammalian cells in culture.丝裂霉素C对培养的哺乳动物细胞的作用。
Biochim Biophys Acta. 1962 Mar 5;55:277-89. doi: 10.1016/0006-3002(62)90783-7.
10
In vitro studies with HeLa cell line sensitive and resistant to actinomycin D.使用对放线菌素D敏感和耐药的HeLa细胞系进行的体外研究。
Ann N Y Acad Sci. 1960 Oct 5;89:474-83. doi: 10.1111/j.1749-6632.1960.tb20171.x.
Zotero 插件